Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3)pLysS cells | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
H225A | the mutant is unstable and precipitates from solution below pH 7.0, decreased activity compared to the wild type enzyme | Pyrococcus furiosus |
H225Q | the mutant displays activity down to solution pH 6.0, increased activity compared to the wild type enzyme | Pyrococcus furiosus |
Y251F | wild type pH stability, increased activity compared to the wild type enzyme | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.95 | - |
L-proline | mutant enzyme Y215F, in 100 mM potassium phosphate buffer, pH 7.5, at 60°C | Pyrococcus furiosus | |
5.3 | - |
L-proline | wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 50°C | Pyrococcus furiosus | |
5.6 | - |
L-proline | wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 40°C | Pyrococcus furiosus | |
9.9 | - |
L-proline | wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 60°C | Pyrococcus furiosus | |
14.46 | - |
L-proline | mutant enzyme H225Q, in 100 mM potassium phosphate buffer, pH 7.5, at 60°C | Pyrococcus furiosus | |
19.67 | - |
L-proline | mutant enzyme H225A, in 100 mM potassium phosphate buffer, pH 7.5, at 60°C | Pyrococcus furiosus | |
19.9 | - |
L-proline | wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 70°C | Pyrococcus furiosus | |
30.8 | - |
L-proline | wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 80°C | Pyrococcus furiosus | |
212.3 | - |
L-pipecolic acid | wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 80°C | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Pyrococcus furiosus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42440 | - |
beta subunit, MALDI-TOF mass spectrometry | Pyrococcus furiosus |
42470 | - |
beta subunit, electrospray ionization mass spectrometry | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
strain DSM 3638 | - |
Purification (Comment) | Organism |
---|---|
DE52 anion exchange column chromatography | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-pipecolic acid + acceptor + H2O | - |
Pyrococcus furiosus | ? + reduced acceptor | - |
? | |
L-proline + acceptor + H2O | preferred substrate | Pyrococcus furiosus | (S)-1-pyrroline-5-carboxylate + reduced acceptor | - |
? | |
additional information | does not reduce NAD+ | Pyrococcus furiosus | ? | - |
? | |
sarcosine + acceptor + H2O | - |
Pyrococcus furiosus | ? + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterooctamer | x-ray crystallography | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
L-proline dehydrogenase | - |
Pyrococcus furiosus |
PRODH | - |
Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
no loss of activity being evident up to 100°C, above this temperature, thermal denaturation of PRODH is apparent, with complete loss of activity after 10 min of incubation in glycerol buffer at temperatures above 115°C | Pyrococcus furiosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
L-pipecolic acid | wild type enzyme in 100 mM potassium phosphate buffer, pH 7.5, at 80°C | Pyrococcus furiosus | |
0.4 | - |
L-proline | wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 40°C | Pyrococcus furiosus | |
1.3 | - |
L-proline | wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 50°C | Pyrococcus furiosus | |
1.4 | - |
L-proline | mutant enzyme H225A, in 100 mM potassium phosphate buffer, pH 7.5, at 60°C | Pyrococcus furiosus | |
4 | - |
L-proline | wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 60°C | Pyrococcus furiosus | |
8.97 | - |
L-proline | mutant enzyme H225Q, in 100 mM potassium phosphate buffer, pH 7.5, at 60°C | Pyrococcus furiosus | |
10.4 | - |
L-proline | wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 70°C | Pyrococcus furiosus | |
18.01 | - |
L-proline | wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 80°C | Pyrococcus furiosus | |
37.17 | - |
L-proline | mutant enzyme Y215F, in 100 mM potassium phosphate buffer, pH 7.5, at 60°C | Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | - |
Pyrococcus furiosus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | - |
Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | the holoenzyme contains one ATP per alphabeta complex | Pyrococcus furiosus | |
FAD | flavoprotein amine dehydrogenase, the holoenzyme contains one FAD per alphabeta complex | Pyrococcus furiosus | |
FMN | the holoenzyme contains one FMN per alphabeta complex | Pyrococcus furiosus |