KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
2,6-dichlorophenolindophenol | - |
bacterium AT2 | |
0.333 | - |
N-Methyl-L-glutamate | - |
bacterium AT2 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
bacterium AT2 | 5829 | - |
membrane | - |
Pseudomonas sp. | 16020 | - |
membrane | - |
Aminobacter aminovorans | 16020 | - |
membrane | - |
bacterium AT2 | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
108000 | - |
4 * 108000 | bacterium AT2 |
407000 | - |
gel filtration | bacterium AT2 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-methyl-L-glutamate + acceptor + H2O | Pseudomonas sp. | - |
L-glutamate + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-glutamate + acceptor + H2O | Aminobacter aminovorans | - |
L-glutamate + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-glutamate + acceptor + H2O | Pseudomonas methylica | - |
L-glutamate + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-glutamate + acceptor + H2O | Hyphomicrobium vulgare | - |
L-glutamate + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-glutamate + acceptor + H2O | bacterium AT2 | - |
L-glutamate + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-glutamate + acceptor + H2O | Hyphomicrobium vulgare ZV | - |
L-glutamate + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-glutamate + acceptor + H2O | Hyphomicrobium vulgare 3 | - |
L-glutamate + formaldehyde + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aminobacter aminovorans | - |
- |
- |
Aminobacter aminovorans AM1 | - |
- |
- |
bacterium AT2 | - |
pink facultative methylotroph | - |
Hyphomicrobium vulgare | - |
- |
- |
Hyphomicrobium vulgare 3 | - |
- |
- |
Hyphomicrobium vulgare ZV | - |
- |
- |
Pseudomonas methylica | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. MA / M.A. / ATCC 23819 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
bacterium AT2 |
partial | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.009 | - |
specific activity after growth on trimethylamine | bacterium AT2 |
0.0161 | - |
- |
Hyphomicrobium vulgare |
0.02 | - |
- |
Aminobacter aminovorans |
0.027 | - |
specific activity after growth on methylamine | bacterium AT2 |
0.0437 | - |
purified enzyme | bacterium AT2 |
0.113 | - |
- |
bacterium AT2 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | - |
Pseudomonas sp. | ? | - |
? | |
additional information | - |
Aminobacter aminovorans | ? | - |
? | |
additional information | not active with NAD+, ferricyanide cytochrome c or horse heart cytochrome c, no activity detected with N-methyl-D-alanine | bacterium AT2 | ? | - |
? | |
additional information | - |
Aminobacter aminovorans AM1 | ? | - |
? | |
additional information | - |
Pseudomonas sp. MA / M.A. / ATCC 23819 | ? | - |
? | |
N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans | DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O | - |
bacterium AT2 | DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans | DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O | - |
bacterium AT2 | DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans AM1 | DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O | - |
Pseudomonas sp. | L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans | L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O | - |
bacterium AT2 | L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans AM1 | L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O | - |
Pseudomonas sp. MA / M.A. / ATCC 23819 | L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methyl-DL-glutamate + 2,6-dichlorophenolindophenol + H2O | - |
bacterium AT2 | DL-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methyl-L-glutamate + acceptor + H2O | - |
Pseudomonas sp. | L-glutamate + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-glutamate + acceptor + H2O | - |
Aminobacter aminovorans | L-glutamate + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-glutamate + acceptor + H2O | - |
Pseudomonas methylica | L-glutamate + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-glutamate + acceptor + H2O | - |
Hyphomicrobium vulgare | L-glutamate + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-glutamate + acceptor + H2O | - |
bacterium AT2 | L-glutamate + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-glutamate + acceptor + H2O | - |
Hyphomicrobium vulgare ZV | L-glutamate + formaldehyde + reduced acceptor | - |
? | |
N-methyl-L-glutamate + acceptor + H2O | - |
Hyphomicrobium vulgare 3 | L-glutamate + formaldehyde + reduced acceptor | - |
? | |
N-methylglutamate + N,N,N,N-tetramethyl-o-phenylene diamine + H2O | - |
bacterium AT2 | L-glutamate + formaldehyde + reduced N,N,N,N-tetramethyl-o-phenylene diamine | - |
? | |
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O | - |
Pseudomonas sp. | L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans | L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O | - |
bacterium AT2 | L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans AM1 | L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O | - |
Pseudomonas sp. MA / M.A. / ATCC 23819 | L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-methylglutamate + phenazine methosulfate + H2O | - |
Pseudomonas sp. | L-glutamate + formaldehyde + reduced phenazine methosulfate | - |
? | |
N-methylglutamate + phenazine methosulfate + H2O | - |
Aminobacter aminovorans | L-glutamate + formaldehyde + reduced phenazine methosulfate | - |
? | |
N-methylglutamate + phenazine methosulfate + H2O | - |
bacterium AT2 | L-glutamate + formaldehyde + reduced phenazine methosulfate | - |
? | |
N-methylglutamate + phenazine methosulfate + H2O | - |
Aminobacter aminovorans AM1 | L-glutamate + formaldehyde + reduced phenazine methosulfate | - |
? | |
N-methylglutamate + phenazine methosulfate + H2O | - |
Pseudomonas sp. MA / M.A. / ATCC 23819 | L-glutamate + formaldehyde + reduced phenazine methosulfate | - |
? | |
N-methylglutamate + radical cation of 2,2'-azinodi-[3-ethylbenzthiazoline 6-sulfonate] + H2O | - |
bacterium AT2 | L-glutamate + formaldehyde + reduced acceptor | - |
? | |
sarcosine + 2,6-dichlorophenolindophenol + H2O | - |
Pseudomonas sp. | aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
sarcosine + 2,6-dichlorophenolindophenol + H2O | - |
Aminobacter aminovorans | aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
sarcosine + 2,6-dichlorophenolindophenol + H2O | - |
bacterium AT2 | aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? | |
sarcosine + 2,6-dichlorophenolindophenol + H2O | - |
Pseudomonas sp. MA / M.A. / ATCC 23819 | aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Aminobacter aminovorans |
tetramer | 4 * 108000 | bacterium AT2 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | half-life of the purified enzyme 35 min at 40°C, 10.5 min at 45°C and 1 min at 50°C | bacterium AT2 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
bacterium AT2 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
bacterium AT2 | |
NAD+ | - |
Pseudomonas methylica | |
NAD+ | - |
Hyphomicrobium vulgare |