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Literature summary for 1.5.98.2 extracted from

  • Ma, K.; Thauer, R.K.
    Purification and properties of N5,N10-methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum (strain Marburg) (1990), Eur. J. Biochem., 191, 187-193.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
reduced coenzyme F420
-
Methanothermobacter thermautotrophicus
0.3
-
N5,N10-methylenetetrahydromethanopterin
-
Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
4 * 36000, SDS-PAGE Methanothermobacter thermautotrophicus
38000
-
8 * 38000, SDS-PAGE Methanothermobacter thermautotrophicus
150000
-
gel filtration Methanothermobacter thermautotrophicus
300000
-
gel filtration Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420 Methanothermobacter thermautotrophicus
-
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420
-
r
N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420 Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420
-
r

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-
Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
163
-
-
Methanothermobacter thermautotrophicus
290
-
-
Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420
-
Methanothermobacter thermautotrophicus 5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420
-
r
N5,N10-methylenetetrahydromethanopterin + reduced coenzyme F420
-
Methanothermobacter thermautotrophicus Marburg / DSM 2133 5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme F420
-
r

Subunits

Subunits Comment Organism
octamer 8 * 38000, SDS-PAGE Methanothermobacter thermautotrophicus
tetramer 4 * 36000, SDS-PAGE Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Methanothermobacter thermautotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
inactivation after a few min, increased thermostability when salts are added Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Methanothermobacter thermautotrophicus
7
-
-
Methanothermobacter thermautotrophicus