KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
coenzyme F420 | - |
Methanopyrus kandleri | |
0.08 | - |
5,10-methylenetetrahydromethanopterin | - |
Methanopyrus kandleri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | very high salt concentration are required for high activity. Highest specific acitivity in presence of 2 M (NH4)2SO4. Other salts including NaH2PO4, KH2PO4 and NaCl are also effective in stimulating the enzyme activity | Methanopyrus kandleri | |
K+ | very high salt concentration is required for high activity. Highest specific acitivity in presence of 2 M (NH4)2SO4. Other salts including NaH2PO4, KH2PO4 and NaCl are also effective in stimulating the enzyme activity | Methanopyrus kandleri | |
Na+ | very high salt concentration is required for high activity. Highest specific acitivity in presence of 2 M (NH4)2SO4. Other salts including NaH2PO4, KH2PO4 and NaCl are also effective in stimulating the enzyme activity | Methanopyrus kandleri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
8 * 36000, SDS-PAGE | Methanopyrus kandleri |
300000 | - |
gel filtration | Methanopyrus kandleri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanopyrus kandleri | - |
- |
- |
Oxidation Stability | Organism |
---|---|
moderately stable against inactivation by air. 50% loss of activity within 12 h at 4°C under aerobic conditions | Methanopyrus kandleri |
Purification (Comment) | Organism |
---|---|
- |
Methanopyrus kandleri |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 + H+ = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | ternary complex mechanism | Methanopyrus kandleri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1320 | - |
- |
Methanopyrus kandleri |
Storage Stability | Organism |
---|---|
-20°C, anaerobically stored, 50% loss of activity after several weeks | Methanopyrus kandleri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | absolutely dependent on coenzyme F420 | Methanopyrus kandleri | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 36000, SDS-PAGE | Methanopyrus kandleri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Methanopyrus kandleri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
stable for 60 min in 50 mM potassium phosphate buffer, pH 6.8. Less than 50% loss of activity after 1 h in deionized water | Methanopyrus kandleri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6.5 | - |
Methanopyrus kandleri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme F420 | required | Methanopyrus kandleri |