Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells as a C-terminally 6-His-tagged fusion protein which is predominantly in its apo form | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
93000 | - |
His-tagged mMe2GlyDH, SDS-PAGE | Rattus norvegicus |
94000 | - |
His-tagged pMe2GlyDH, SDS-PAGE | Rattus norvegicus |
97410 | - |
pMe2GlyDH, calculated from amino acid sequence | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q63342 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-Sepharose column chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N-dimethylglycine + acceptor + H2O | - |
Rattus norvegicus | sarcosine + formaldehyde + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mMe2GlyDH | mature dimethylglycine dehydrogenase | Rattus norvegicus |
pMe2GlyDH | precursor of dimethylglycine dehydrogenase | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FAD is covalently bound via an autocatalytic reaction | Rattus norvegicus |