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Literature summary for 1.5.8.3 extracted from
- Purification and some properties of sarcosine dehydrogenase from Pseudomonas putida RS65 (2001), Food Sci. Agric. Chem., 3, 36-41.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM | Pseudomonas putida |  |
| Cu2+ | not in | Pseudomonas putida | |
| Hg2+ | 1 mM | Pseudomonas putida | |
| additional information | not Co2+, Na+, EDTA, SDS, Triton X-100, semicarbazide; not inhibited by: Ba2+, Ca2+, Fe3+, Li+, Zn2+, dithiothreitol, 2-mercaptoethanol, iodoacetate | Pseudomonas putida | |
| PCMB | 2 mM, 100% loss of activity | Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.15 | - |
N-Methylalanine | - |
Pseudomonas putida | |
| 3.9 | - |
sarcosine | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 107000 | - |
SDS-PAGE | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sarcosine + acceptor + H2O | Pseudomonas putida | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate | glycine + formaldehyde + reduced acceptor | - |
? | |
| sarcosine + acceptor + H2O | Pseudomonas putida RS65 | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate | glycine + formaldehyde + reduced acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
RS65 | - |
| Pseudomonas putida RS65 | - |
RS65 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-methyl-L-alanine + acceptor + H2O | - |
Pseudomonas putida | L-alanine + formaldehyde + reduced acceptor | - |
? | |
| N-methyl-L-alanine + acceptor + H2O | - |
Pseudomonas putida RS65 | L-alanine + formaldehyde + reduced acceptor | - |
? | |
| sarcosine + acceptor + H2O | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate | Pseudomonas putida | glycine + formaldehyde + reduced acceptor | - |
? | |
| sarcosine + acceptor + H2O | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate | Pseudomonas putida RS65 | glycine + formaldehyde + reduced acceptor | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pseudomonas putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Pseudomonas putida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | at 25°C for 6 h | Pseudomonas putida |
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