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Literature summary for 1.5.8.2 extracted from

  • Liffourrena, A.S.; Salvano, M.A.; Lucchesi, G.I.
    Pseudomonas putida A ATCC 12633 oxidizes trimethylamine aerobically via two different pathways (2010), Arch. Microbiol., 192, 471-476.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is not activated by AlCl3 Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
trimethylamine substrate inhibition Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
trimethylamine pH 7.4, 30°C Pseudomonas putida
4
-
trimethylamine pH not specified in the publication, temperature not specified in the publication Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trimethylamine + H2O + electron-transfer flavoprotein Pseudomonas putida
-
dimethylamine + formaldehyde + reduced electron-transferring flavoprotein
-
?
trimethylamine + H2O + electron-transfer flavoprotein Pseudomonas putida ATCC 12633
-
dimethylamine + formaldehyde + reduced electron-transferring flavoprotein
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida
-
strain is able to grow on trimethylamine as a nitrogen source and trimethylamine also accumulates in the cell, slowing the bacterial growth
-
Pseudomonas putida ATCC 12633
-
-
-
Pseudomonas putida ATCC 12633
-
strain is able to grow on trimethylamine as a nitrogen source and trimethylamine also accumulates in the cell, slowing the bacterial growth
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:tetradecyltrimethylammonium bromide-grown cell
-
Pseudomonas putida
-
additional information no activity in cells grown on trimethylamine Pseudomonas putida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000031
-
tetradecyltrimethylammonium bromide-grown cells, pH 7.4, 30°C Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trimethylamine + H2O + electron acceptor
-
Pseudomonas putida dimethylamine + formaldehyde + reduced electron acceptor
-
?
trimethylamine + H2O + electron acceptor
-
Pseudomonas putida ATCC 12633 dimethylamine + formaldehyde + reduced electron acceptor
-
?
trimethylamine + H2O + electron-transfer flavoprotein
-
Pseudomonas putida dimethylamine + formaldehyde + reduced electron-transferring flavoprotein
-
?
trimethylamine + H2O + electron-transfer flavoprotein
-
Pseudomonas putida ATCC 12633 dimethylamine + formaldehyde + reduced electron-transferring flavoprotein
-
?

Synonyms

Synonyms Comment Organism
TMA dehydrogenase
-
Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
additional information independent of NADPH Pseudomonas putida