Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-phenylethylhydrazine | potent inhibition at very low concentration | methylotrophic bacterium | |
2-phenylcyclopropylamine | 82% inhibition at 0.1 mM | methylotrophic bacterium | |
2-phenylethylhydrazine | potent inhibition at very low concentration | methylotrophic bacterium | |
Ag+ | strong inhibition at 1 mM | methylotrophic bacterium | |
benzylhydrazine | potent inhibition at very low concentration | methylotrophic bacterium | |
Betaine hydrochloride | 37% inhibition at 1 mM | methylotrophic bacterium | |
choline chloride | potent inhibition at 1 mM | methylotrophic bacterium | |
Co2+ | strong inhibition at 1 mM | methylotrophic bacterium | |
Cu2+ | strong inhibition at 1 mM | methylotrophic bacterium | |
Harmaline | 65% inhibition at 0.1 mM | methylotrophic bacterium | |
hexamethylene bis-trimethylammonium chloride | potent inhibition at 1 mM | methylotrophic bacterium | |
Hg2+ | strong inhibition at 1 mM | methylotrophic bacterium | |
iodoacetamide | inhibition at 1 mM | methylotrophic bacterium | |
Iproniazid | potent inhibition at very low concentration | methylotrophic bacterium | |
isocarboxazid | potent inhibition at very low concentration | methylotrophic bacterium | |
isopropylhydrazine | potent inhibition at very low concentration | methylotrophic bacterium | |
N,N,N-trimethyl-N-phenylammonium chloride | potent inhibition at 1 mM | methylotrophic bacterium | |
N,N,N-trimethylhydrazonium iodide | potent inhibition at 1 mM | methylotrophic bacterium | |
N-benzyl-N-methylpropargylamine | 27% inhibition at 0.1 mM | methylotrophic bacterium | |
N-ethylmaleimide | inhibition at 1 mM | methylotrophic bacterium | |
Ni2+ | strong inhibition at 1 mM | methylotrophic bacterium | |
nialamid | potent inhibition at very low concentration | methylotrophic bacterium | |
p-chloromercuribenzoate | inhibition at 1 mM | methylotrophic bacterium | |
phenylhydrazine | potent inhibition at very low concentration | methylotrophic bacterium | |
Tetramethylammonium chloride | potent inhibition at 1 mM | methylotrophic bacterium | |
trimethylamine | inhibitory at higher concentrations | methylotrophic bacterium | |
trimethylsulfomium chloride | potent inhibition at 1 mM | methylotrophic bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
trimethylamine | electron acceptor: phenazine methosulfate | methylotrophic bacterium | |
0.002 | - |
ethyldimethylamine | electron acceptor: phenazine methosulfate | methylotrophic bacterium | |
0.002 | - |
2-chloroethyldimethylamine | electron acceptor: phenazine methosulfate | methylotrophic bacterium | |
0.006 | - |
2-hydroxyethyldimethylamine | electron acceptor: phenazine methosulfate | methylotrophic bacterium | |
0.008 | - |
diethylmethylamine | electron acceptor: phenazine methosulfate | methylotrophic bacterium | |
0.067 | - |
2-aminoethyldimethylamine | electron acceptor: phenazine methosulfate | methylotrophic bacterium | |
0.13 | - |
triethylamine | electron acceptor: phenazine methosulfate | methylotrophic bacterium | |
1.25 | - |
phenazine methosulfate | substrate: trimethylamine | methylotrophic bacterium | |
1.7 | - |
Diethylamine | electron acceptor: phenazine methosulfate | methylotrophic bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
161000 | - |
gel filtration | methylotrophic bacterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trimethylamine + H2O + electron acceptor | methylotrophic bacterium | electron acceptors: phenazine methosulfate, brilliant cresyl blue, methylene blue | dimethylamine + formaldehyde + reduced electron acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
methylotrophic bacterium | - |
- |
- |
methylotrophic bacterium 4B6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | methylotrophic bacterium |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
methylotrophic bacterium | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
- |
methylotrophic bacterium |
Storage Stability | Organism |
---|---|
-20°C, 20 mM sodium phosphate, pH 7.5, 0.4 mg protein/ml, stable for 3 months | methylotrophic bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminoethyldimethylamine + H2O + phenazine methosulfate | 10% of the activity compared to trimethylamine | methylotrophic bacterium | ? + reduced phenazine methosulfate | - |
? | |
2-aminoethyldimethylamine + H2O + phenazine methosulfate | 10% of the activity compared to trimethylamine | methylotrophic bacterium 4B6 | ? + reduced phenazine methosulfate | - |
? | |
2-chloroethyldimethylamine + H2O + phenazine methosulfate | 40% of the activity compared to trimethylamine | methylotrophic bacterium | ? + reduced phenazine methosulfate | - |
? | |
2-hydroxyethyldimethylamine + H2O + phenazine methosulfate | 70% of the activity compared to trimethylamine | methylotrophic bacterium | ? + reduced phenazine methosulfate | - |
? | |
diethylamine + H2O + phenazine methosulfate | 47% of the activity compared to trimethylamine | methylotrophic bacterium | ethylamine + acetaldehyde + reduced phenazine methosulfate | - |
? | |
diethylamine + H2O + phenazine methosulfate | 47% of the activity compared to trimethylamine | methylotrophic bacterium 4B6 | ethylamine + acetaldehyde + reduced phenazine methosulfate | - |
? | |
diethylmethylamine + H2O + phenazine methosulfate | 11% of the activity compared to trimethylamine | methylotrophic bacterium | diethylamine + ethylmethylamine + formaldehyde + acetaldehyde + reduced phenazine methosulfate | - |
? | |
diethylmethylamine + H2O + phenazine methosulfate | 11% of the activity compared to trimethylamine | methylotrophic bacterium 4B6 | diethylamine + ethylmethylamine + formaldehyde + acetaldehyde + reduced phenazine methosulfate | - |
? | |
ethyldimethylamine + H2O + phenazine methosulfate | 16% of the activity compared to trimethylamine | methylotrophic bacterium | ethylmethylamine + formaldehyde + reduced phenazine methosulfate | - |
? | |
ethyldimethylamine + H2O + phenazine methosulfate | 16% of the activity compared to trimethylamine | methylotrophic bacterium 4B6 | ethylmethylamine + formaldehyde + reduced phenazine methosulfate | - |
? | |
triethylamine + H2O + phenazine methosulfate | 3% of the activity compared to trimethylamine | methylotrophic bacterium | diethylamine + acetaldehyde + reduced phenazine methosulfate | - |
? | |
trimethylamine + H2O + electron acceptor | electron acceptors: phenazine methosulfate, brilliant cresyl blue, methylene blue | methylotrophic bacterium | dimethylamine + formaldehyde + reduced electron acceptor | - |
? | |
trimethylamine + H2O + phenazine methosulfate | - |
methylotrophic bacterium | dimethylamine + formaldehyde + reduced phenazine methosulfate | - |
? | |
trimethylamine + H2O + phenazine methosulfate | - |
methylotrophic bacterium 4B6 | dimethylamine + formaldehyde + reduced phenazine methosulfate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
loss of 50% of activity in 20 min | methylotrophic bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
methylotrophic bacterium |