Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methylophilus methylotrophus |
Protein Variants | Comment | Organism |
---|---|---|
Y442F | 1.4fold reduction of electron transfer | Methylophilus methylotrophus |
Y442G | 30.5fold reduction of electron transfer | Methylophilus methylotrophus |
Y442L | 2.2fold reduction of electron transfer | Methylophilus methylotrophus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0143 | - |
trimethylamine | mutant Y442F, electron acceptor: electron transferring flavoprotein | Methylophilus methylotrophus | |
0.0175 | - |
trimethylamine | wild-type form, electron acceptor: electron transferring flavoprotein | Methylophilus methylotrophus | |
0.0196 | - |
trimethylamine | mutant Y442L, electron acceptor: electron transferring flavoprotein | Methylophilus methylotrophus | |
0.0262 | - |
trimethylamine | mutant Y442G, electron acceptor: electron transferring flavoprotein | Methylophilus methylotrophus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 4Fe-4S-center | Methylophilus methylotrophus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trimethylamine + H2O + electron transferring flavoprotein | Methylophilus methylotrophus | - |
dimethylamine + formaldehyde + reduced electron transferring flavoprotein | - |
? | |
trimethylamine + H2O + electron transferring flavoprotein | Methylophilus methylotrophus W3A1 | - |
dimethylamine + formaldehyde + reduced electron transferring flavoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylophilus methylotrophus | - |
- |
- |
Methylophilus methylotrophus W3A1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Methylophilus methylotrophus |
to homogeneity, recombinant enzyme | Methylophilus methylotrophus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Methylophilus methylotrophus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trimethylamine + H2O + electron transferring flavoprotein | - |
Methylophilus methylotrophus | dimethylamine + formaldehyde + reduced electron transferring flavoprotein | - |
? | |
trimethylamine + H2O + electron transferring flavoprotein | - |
Methylophilus methylotrophus W3A1 | dimethylamine + formaldehyde + reduced electron transferring flavoprotein | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
trimethylamine | mutant Y442G, electron acceptor: electron transferring flavoprotein | Methylophilus methylotrophus | |
3.8 | - |
trimethylamine | mutant Y442L, electron acceptor: electron transferring flavoprotein | Methylophilus methylotrophus | |
10.3 | - |
trimethylamine | mutant Y442F, electron acceptor: electron transferring flavoprotein | Methylophilus methylotrophus | |
16.5 | - |
trimethylamine | wild-type form, electron acceptor: electron transferring flavoprotein | Methylophilus methylotrophus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Methylophilus methylotrophus |