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Literature summary for 1.5.5.2 extracted from

  • Ostrander, E.L.; Larson, J.D.; Schuermann, J.P.; Tanner, J.J.
    A conserved active site tyrosine residue of proline dehydrogenase helps enforce the preference for proline over hydroxyproline as the substrate (2009), Biochemistry, 48, 951-959.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.5.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant Y540S Escherichia coli

Protein Variants

Protein Variants Comment Organism
Y540A a construct consisting of residue 86-630 of PutA is used Escherichia coli
Y540S a construct consisting of residue 86-630 of PutA is used Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
L-tetrahydro-2-furoic acid
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5 20 L-proline Y540A mutant Escherichia coli
59
-
 L-proline wild type Escherichia coli
100
-
 L-proline Y540S mutant Escherichia coli
190
-
 trans-4-hydroxy-L-proline Y540S mutant Escherichia coli
310
-
 trans-4-hydroxy-L-proline Y540A mutant Escherichia coli
750
-
 trans-4-hydroxy-L-proline wild type Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P09546
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-proline + oxidized dichlorophenolindophenol
-
 Escherichia coli 1-pyrroline-5-carboxylate + reduced dichlorophenolindophenol
-
 ?
trans-4-hydroxy-L-proline + oxidized dichlorophenolindophenol
-
 Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
proline dehydrogenase bifunctional enzyme: proline utilization A (PutA) Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 trans-4-hydroxy-L-proline Y540S mutant Escherichia coli
2.5
-
 trans-4-hydroxy-L-proline wild type Escherichia coli
2.9
-
 trans-4-hydroxy-L-proline Y540A mutant Escherichia coli
7.8
-
 L-proline Y540S mutant Escherichia coli
13
-
 L-proline Y540A mutant Escherichia coli
29
-
 L-proline wild type Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.084
-
 L-tetrahydro-2-furoic acid
-
 Escherichia coli
1.5
-
 L-tetrahydro-2-furoic acid mutant Y540S Escherichia coli
7.9
-
 L-tetrahydro-2-furoic acid mutant Y540A Escherichia coli