Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.3.6 extracted from

  • Brandsch, R.; Bichler, V.
    Autoflavinylation of apo6-hydroxy-D-nicotine oxidase (1991), J. Biol. Chem., 266, 19056-19062.
    View publication on PubMed
Search for more literature for 1.5.3.6

Activating Compound

Activating Compound Comment Organism Structure
glycerol activation of autocatalytical flavinylation of the enzyme at 45% v/v Paenarthrobacter nicotinovorans
sucrose activation of autocatalytical flavinylation of the enzyme at 20% Paenarthrobacter nicotinovorans

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 as a beta-galactosidase/6-hydroxynicotine oxidase fusion protein Paenarthrobacter nicotinovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-6-hydroxynicotine + H2O + O2 Paenarthrobacter nicotinovorans
-
 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Paenarthrobacter nicotinovorans
-
 formerly Arthrobacter oxidans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-6-hydroxynicotine + H2O + O2
-
 Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 the ability of building the holoenzyme is abolished in absence of glycerol Paenarthrobacter nicotinovorans

Cofactor

Cofactor Comment Organism Structure
FAD formation of the bond to FAD proceeds autocatalytically Paenarthrobacter nicotinovorans
FAD covalently bound to a histidine Paenarthrobacter nicotinovorans