General Stability | Organism |
---|---|
10% glycerol protects enzyme from inactivation during Sephadex G-200 column chromatography | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,6-dichlorophenolindophenol | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.05 | - |
N6-methyl-L-lysine | crude extract | Rattus norvegicus | |
1.9 | - |
N6-methyl-L-lysine | partially purified enzyme | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | exclusive localization | Rattus norvegicus | 5739 | - |
particle-bound | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | 200000 | gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
16fold partial purification | Rattus norvegicus |
enzyme from kidney | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N6-methyl-L-lysine + H2O + O2 = L-lysine + formaldehyde + H2O2 | mechanism | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | low enzyme activity in young rat, increases to the adult level, highest level of demethylation activity | Rattus norvegicus | - |
liver | low enzyme activity in young rat, increases to the adult level, after kidney next highest level of demethylation activity | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
values in dependence of different incubation mixtures | Rattus norvegicus |
0.0283 | - |
11fold purified enzyme | Rattus norvegicus |
0.0433 | - |
16fold purified enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-(methylamino)-2-oxohexanoic acid + H2O + O2 | free alpha-amino group is not required for susceptibility | Rattus norvegicus | 6-amino-2-oxohexanoic acid + formaldehyde + H2O2 | - |
? | |
additional information | not: delta-N-monomethyl-L-ornithine | Rattus norvegicus | ? | - |
? | |
additional information | demethylation involves an electron transport system: FAD is the physiological electron acceptor, phenazine methosulfate as artificial and more efficient electron acceptor than FAD, final electron acceptor is oxygen or 2,6-dichlorophenolindophenol | Rattus norvegicus | ? | - |
? | |
N6,N6-dimethyl-L-lysine + O2 + H2O | - |
Rattus norvegicus | L-lysine + formaldehyde + H2O2 | - |
? | |
N6-methyl-D-lysine + H2O + O2 | 11% of the activity with epsilon-N-methyl-L-lysine | Rattus norvegicus | D-lysine + formaldehyde + H2O2 | - |
? | |
N6-methyl-L-lysine + O2 + H2O | reaction in reverse direction, i.e. methylation of L-lysine, is probably catalyzed by protein methylase III | Rattus norvegicus | L-lysine + formaldehyde + H2O2 | equimolar amounts of L-lysine and formaldehyde | ir | |
N6-methyl-L-lysine + O2 + H2O | enzyme consumes per mol N6-methyl-L-lysine half a mol of oxygen | Rattus norvegicus | L-lysine + formaldehyde + H2O2 | equimolar amounts of L-lysine and formaldehyde | ir |
Synonyms | Comment | Organism |
---|---|---|
More | identical to histone demethylase | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
4 min: 70% loss of activity, 10 min: complete loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | pH 6: about 35% of activity maximum, pH 8: about 50% of activity maximum | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | physiological electron acceptor, not required with whole homogenate, but required for partially purified enzyme | Rattus norvegicus |