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Literature summary for 1.5.3.4 extracted from

  • Paik, W.K.; Kim, S.
    epsilon-Alkyllysinase. New assay method, purification, and biological significance (1974), Arch. Biochem. Biophys., 165, 369-378.
    View publication on PubMed

General Stability

General Stability Organism
10% glycerol protects enzyme from inactivation during Sephadex G-200 column chromatography Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2,6-dichlorophenolindophenol
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.05
-
N6-methyl-L-lysine crude extract Rattus norvegicus
1.9
-
N6-methyl-L-lysine partially purified enzyme Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion exclusive localization Rattus norvegicus 5739
-
particle-bound
-
Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000 200000 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
16fold partial purification Rattus norvegicus
enzyme from kidney Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
N6-methyl-L-lysine + H2O + O2 = L-lysine + formaldehyde + H2O2 mechanism Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney low enzyme activity in young rat, increases to the adult level, highest level of demethylation activity Rattus norvegicus
-
liver low enzyme activity in young rat, increases to the adult level, after kidney next highest level of demethylation activity Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
values in dependence of different incubation mixtures Rattus norvegicus
0.0283
-
11fold purified enzyme Rattus norvegicus
0.0433
-
16fold purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-(methylamino)-2-oxohexanoic acid + H2O + O2 free alpha-amino group is not required for susceptibility Rattus norvegicus 6-amino-2-oxohexanoic acid + formaldehyde + H2O2
-
?
additional information not: delta-N-monomethyl-L-ornithine Rattus norvegicus ?
-
?
additional information demethylation involves an electron transport system: FAD is the physiological electron acceptor, phenazine methosulfate as artificial and more efficient electron acceptor than FAD, final electron acceptor is oxygen or 2,6-dichlorophenolindophenol Rattus norvegicus ?
-
?
N6,N6-dimethyl-L-lysine + O2 + H2O
-
Rattus norvegicus L-lysine + formaldehyde + H2O2
-
?
N6-methyl-D-lysine + H2O + O2 11% of the activity with epsilon-N-methyl-L-lysine Rattus norvegicus D-lysine + formaldehyde + H2O2
-
?
N6-methyl-L-lysine + O2 + H2O reaction in reverse direction, i.e. methylation of L-lysine, is probably catalyzed by protein methylase III Rattus norvegicus L-lysine + formaldehyde + H2O2 equimolar amounts of L-lysine and formaldehyde ir
N6-methyl-L-lysine + O2 + H2O enzyme consumes per mol N6-methyl-L-lysine half a mol of oxygen Rattus norvegicus L-lysine + formaldehyde + H2O2 equimolar amounts of L-lysine and formaldehyde ir

Synonyms

Synonyms Comment Organism
More identical to histone demethylase Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
4 min: 70% loss of activity, 10 min: complete loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6: about 35% of activity maximum, pH 8: about 50% of activity maximum Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD physiological electron acceptor, not required with whole homogenate, but required for partially purified enzyme Rattus norvegicus