Application | Comment | Organism |
---|---|---|
analysis | spectrophotometric method for determining glyphospate oxidoreductase activity in cell-free extract based on the rate of glyoxylate hydrazone formation | Brucella anthropi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brucella anthropi | - |
- |
- |
Brucella anthropi GPK 3 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.033 | - |
spectrophotometric determination of product, pH 8.0, 30°C | Brucella anthropi |
0.034 | - |
determination of product by HPLC, pH 8.0, 30°C | Brucella anthropi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 glyphosate + O2 | - |
Brucella anthropi | 2 aminomethylphosphonate + 2 glyoxylate | - |
? | |
2 glyphosate + O2 | - |
Brucella anthropi GPK 3 | 2 aminomethylphosphonate + 2 glyoxylate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Brucella anthropi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Brucella anthropi |
General Information | Comment | Organism |
---|---|---|
physiological function | glyphosate degradation can follow different pathways depending on physiological characteristics of metabolizing strains. In Ochrobactrum anthropi GPK3 the initial cleavage reaction is catalyzed by glyphosate oxidoreductase with the formation of aminomethylphosphonic acid and glyoxylate, whereas Achromobacter sp. MPS12 utilize C-P lyase, forming sarcosine | Brucella anthropi |