Literature summary for 1.5.3.17 extracted from

Samasil, K.; Lopes de Carvalho, L.; Maeenpaeae, P.; Salminen, T.A.; Incharoensakdi, A.
Biochemical characterization and homology modeling of polyamine oxidase from cyanobacterium Synechocystis sp. PCC 6803 (2017), Plant Physiol. Biochem., 119, 159-169 .

Search for more literature for 1.5.3.17

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Synechocystis sp. PCC 6803

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.098	-	spermine	pH 8.5, 30°C	Synechocystis sp. PCC 6803
0.456	-	spermidine	pH 8.5, 30°C	Synechocystis sp. PCC 6803

Organism

Organism	UniProt	Comment	Textmining
Synechocystis sp. PCC 6803	Q6ZEN7	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	substrate preference in decreasing order: spermine, spermidine, N1-acetylspermine	Synechocystis sp. PCC 6803	?	-	-
N1-acetylspermine + O2 + H2O	-	Synechocystis sp. PCC 6803	spermidine + N-acetyl-3-aminopropanal + H2O2	-	?
spermidine + O2 + H2O	-	Synechocystis sp. PCC 6803	putrescine + 3-aminobutanal + H2O2	-	?
spermine + O2 + H2O	-	Synechocystis sp. PCC 6803	spermidine + 3-aminopropanal + H2O2	-	?

Synonyms

Synonyms	Comment	Organism
slr5093	-	Synechocystis sp. PCC 6803

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Synechocystis sp. PCC 6803

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.17	-	spermidine	pH 8.5, 30°C	Synechocystis sp. PCC 6803
0.31	-	spermine	pH 8.5, 30°C	Synechocystis sp. PCC 6803

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Synechocystis sp. PCC 6803

Cofactor

Cofactor	Comment	Organism	Structure
FAD	purified enzyme shows absorbance peaks at 380 and 450 nm	Synechocystis sp. PCC 6803

General Information

General Information	Comment	Organism
metabolism	residues Gln94, Tyr403 and Thr440 are predicted to be important in the active site	Synechocystis sp. PCC 6803

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.37	-	spermidine	pH 8.5, 30°C	Synechocystis sp. PCC 6803
3.16	-	spermine	pH 8.5, 30°C	Synechocystis sp. PCC 6803

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Release 2023.1 (January 2023)