Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes expressed in Escherichia coli | Branchiostoma japonicum |
Protein Variants | Comment | Organism |
---|---|---|
H64E | Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym | Branchiostoma japonicum |
H64Q | Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym | Branchiostoma japonicum |
H69E | compared with the wild-type enzyme the mutant enzyme shows lower Vmax values and higher Km-values for the substrates spermidine and N1-acetylspermine | Branchiostoma japonicum |
H69Q | compared with the wild-type enzyme the mutant enzyme shows lower Vmax values and higher Km-values for the substrates spermidine and N1-acetylspermine | Branchiostoma japonicum |
K301M | Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym | Branchiostoma japonicum |
K315M | compared with the wild-type enzyme the mutant enzyme shows lower Vmax values and higher Km-values for the substrates spermidine and N1-acetylspermine | Branchiostoma japonicum |
T460S | Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym | Branchiostoma japonicum |
T460Y | Vmax values for mutant enzyme with substrate spermine or spermidine are lower than Vmax values for wild-type enzym. Km values for mutant enzyme with substrate spermine or spermidine are higher than Km values for wild-type enzym | Branchiostoma japonicum |
T467S | compared with the wild-type enzyme the mutant enzyme shows lower Vmax values and higher Km-values for the substrates spermidine and N1-acetylspermine | Branchiostoma japonicum |
T467Y | compared with the wild-type enzyme the mutant enzyme shows lower Vmax values and higher Km-values for the substrates spermidine and N1-acetylspermine | Branchiostoma japonicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N1,N4-bis(2,3-butadienyl)-1,4-butanediamine | 2.5 mM, 50% inhibition of spermidine oxidation, 70% inhibition of N1-acetylspermine oxidation; 2.5 mM, 50% inhibition of spermidine oxidation, 70% inhibition of spermine oxidation | Branchiostoma japonicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
spermidine | 37°C, pH 9.5, wild-type enzyme | Branchiostoma japonicum | |
0.00836 | - |
spermine | 37°C, pH 8.5, wild-type enzyme | Branchiostoma japonicum | |
0.012 | - |
spermine | 37°C, pH 8.5, mutant enzyme T460S | Branchiostoma japonicum | |
0.0124 | - |
spermidine | 37°C, pH 9.5, wild-type enzyme | Branchiostoma japonicum | |
0.013 | - |
spermine | 37°C, pH 8.5, mutant enzyme T460Y | Branchiostoma japonicum | |
0.016 | - |
spermidine | 37°C, pH 9.5, mutant enzyme K301M | Branchiostoma japonicum | |
0.017 | - |
spermine | 37°C, pH 8.5, mutant enzyme K301M | Branchiostoma japonicum | |
0.017 | - |
spermidine | 37°C, pH 9.5, mutant enzyme T460Y | Branchiostoma japonicum | |
0.019 | - |
spermidine | 37°C, pH 9.5, mutant enzyme H64E | Branchiostoma japonicum | |
0.019 | - |
spermidine | 37°C, pH 9.5, mutant enzyme T460S | Branchiostoma japonicum | |
0.02 | - |
spermidine | 37°C, pH 9.5, mutant enzyme T467Y | Branchiostoma japonicum | |
0.021 | - |
spermidine | 37°C, pH 9.5, mutant enzyme H64Q | Branchiostoma japonicum | |
0.0229 | - |
spermidine | 37°C, pH 9.5, mutant enzyme H69Q | Branchiostoma japonicum | |
0.024 | - |
spermine | 37°C, pH 8.5, mutant enzyme H64E | Branchiostoma japonicum | |
0.025 | - |
spermidine | 37°C, pH 9.5, mutant enzyme H69E | Branchiostoma japonicum | |
0.0269 | - |
spermine | 37°C, pH 8.5, mutant enzyme H64Q | Branchiostoma japonicum | |
0.031 | - |
spermidine | 37°C, pH 9.5, mutant enzyme K315M | Branchiostoma japonicum | |
0.0369 | - |
N1-acetylspermine | 37°C, pH 8.0, wild-type enzyme | Branchiostoma japonicum | |
0.043 | - |
N1-acetylspermine | 37°C, pH 8.0, mutant enzyme K315M | Branchiostoma japonicum | |
0.046 | - |
N1-acetylspermine | 37°C, pH 8.0, mutant enzyme T467Y | Branchiostoma japonicum | |
0.058 | - |
N1-acetylspermine | 37°C, pH 8.0, mutant enzyme T467S | Branchiostoma japonicum | |
0.062 | - |
N1-acetylspermine | 37°C, pH 8.0, mutant enzyme H69E | Branchiostoma japonicum | |
0.071 | - |
N1-acetylspermine | 37°C, pH 8.0, mutant enzyme H69Q | Branchiostoma japonicum | |
0.182 | - |
spermidine | 37°C, pH 9.5, mutant enzyme T467S | Branchiostoma japonicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Branchiostoma japonicum | - |
- |
- |
Branchiostoma japonicum | A0A0N7IZL9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Branchiostoma japonicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gill | - |
Branchiostoma japonicum | - |
hepatic cecum | - |
Branchiostoma japonicum | - |
hindgut | - |
Branchiostoma japonicum | - |
muscle | - |
Branchiostoma japonicum | - |
notochord | - |
Branchiostoma japonicum | - |
notochord | Bjpao1 is predominantly expressed in the notochord, testis and ovary | Branchiostoma japonicum | - |
ovary | - |
Branchiostoma japonicum | - |
ovary | Bjpao1 is predominantly expressed in the notochord, testis and ovary | Branchiostoma japonicum | - |
testis | - |
Branchiostoma japonicum | - |
testis | Bjpao1 is predominantly expressed in the notochord, testis and ovary | Branchiostoma japonicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bjpao1 shows no activity with N1-acetylspermine | Branchiostoma japonicum | ? | - |
? | |
additional information | Bjpao2 shows no activity with spermine | Branchiostoma japonicum | ? | - |
? | |
N1-acetylspermine + O2 + H2O | - |
Branchiostoma japonicum | spermidine + 3-acetamidopropanal + H2O2 | - |
? | |
spermidine + O2 + H2O | - |
Branchiostoma japonicum | putrescine + 3-aminopropanal + H2O2 | - |
? | |
spermine + O2 + H2O | - |
Branchiostoma japonicum | spermidine + 3-aminopropanal + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, SDS-PAGE | Branchiostoma japonicum |
? | x * 59000, SDS-PAGE | Branchiostoma japonicum |
Synonyms | Comment | Organism |
---|---|---|
Bjpao1 | - |
Branchiostoma japonicum |
Bjpao2 | - |
Branchiostoma japonicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 37 | - |
Branchiostoma japonicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
substrate: N1-acetylspermine | Branchiostoma japonicum |
8.5 | - |
substrate: spermine | Branchiostoma japonicum |
9 | - |
substrate: spermidine | Branchiostoma japonicum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Branchiostoma japonicum | calculated from sequence | - |
4.9 |
Branchiostoma japonicum | calculated from sequence | - |
5.13 |