Cloned (Comment) | Organism |
---|---|
ZmPAO, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, expression of His-tagged enzyme in Pichia pastoris | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
spermine | pH 7.5, 25°C, recombinant ZmPAO | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
spermidine + O2 + H2O | Zea mays | - |
propane-1,3-diamine + 4-aminobutanal + H2O2 | - |
? | |
spermine + O2 + H2O | Zea mays | - |
propane-1,3-diamine + N-(3-aminopropyl)-4-aminobutanal + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Pichia pastoris strain X-33 by nickel affinity chromatography | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the aminoaldehydes 4-aminobutanal and N-(3-aminopropyl)-4-aminobutanal are produced during ZmPAO reaction with spermidine and spermine, respectively | Zea mays | ? | - |
? | |
spermidine + O2 + H2O | - |
Zea mays | propane-1,3-diamine + 4-aminobutanal + H2O2 | - |
? | |
spermine + O2 + H2O | - |
Zea mays | propane-1,3-diamine + N-(3-aminopropyl)-4-aminobutanal + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ZmPAO | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32.9 | - |
spermine | pH 7.5, 25°C, recombinant ZmPAO | Zea mays | |
50.2 | - |
spermidine | pH 7.5, 25°C, recombinant AtPAO2 | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent on | Zea mays |
General Information | Comment | Organism |
---|---|---|
physiological function | plant PAOs oxidize the carbon at the endo-side of the N4-nitrogen of Spd and Spm, producing 4-aminobutanal and N-(3-aminopropyl)-4-aminobutanal, respectively, in addition to 1,3-diaminopropane and H2O2, and are considered to be involved in terminal catabolism of polyamines, physiological role(s) of the polyamine catabolic pathways in plants, overview | Zea mays |