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Literature summary for 1.5.3.14 extracted from

  • Fincato, P.; Moschou, P.N.; Spedaletti, V.; Tavazza, R.; Angelini, R.; Federico, R.; Roubelakis-Angelakis, K.A.; Tavladoraki, P.
    Functional diversity inside the Arabidopsis polyamine oxidase gene family (2011), J. Exp. Bot., 62, 1155-1168.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
ZmPAO, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, expression of His-tagged enzyme in Pichia pastoris Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
spermine pH 7.5, 25°C, recombinant ZmPAO Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
spermidine + O2 + H2O Zea mays
-
propane-1,3-diamine + 4-aminobutanal + H2O2
-
?
spermine + O2 + H2O Zea mays
-
propane-1,3-diamine + N-(3-aminopropyl)-4-aminobutanal + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Pichia pastoris strain X-33 by nickel affinity chromatography Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the aminoaldehydes 4-aminobutanal and N-(3-aminopropyl)-4-aminobutanal are produced during ZmPAO reaction with spermidine and spermine, respectively Zea mays ?
-
?
spermidine + O2 + H2O
-
Zea mays propane-1,3-diamine + 4-aminobutanal + H2O2
-
?
spermine + O2 + H2O
-
Zea mays propane-1,3-diamine + N-(3-aminopropyl)-4-aminobutanal + H2O2
-
?

Synonyms

Synonyms Comment Organism
ZmPAO
-
Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
32.9
-
spermine pH 7.5, 25°C, recombinant ZmPAO Zea mays
50.2
-
spermidine pH 7.5, 25°C, recombinant AtPAO2 Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Zea mays

Cofactor

Cofactor Comment Organism Structure
FAD dependent on Zea mays

General Information

General Information Comment Organism
physiological function plant PAOs oxidize the carbon at the endo-side of the N4-nitrogen of Spd and Spm, producing 4-aminobutanal and N-(3-aminopropyl)-4-aminobutanal, respectively, in addition to 1,3-diaminopropane and H2O2, and are considered to be involved in terminal catabolism of polyamines, physiological role(s) of the polyamine catabolic pathways in plants, overview Zea mays