Protein Variants | Comment | Organism |
---|---|---|
K171A | the mutant shows 39% activity of the wild type enzyme | Corynebacterium sp. U-96 |
K171D | the mutant shows 32% activity of the wild type enzyme | Corynebacterium sp. U-96 |
K171R | the mutant shows 58% activity of the wild type enzyme | Corynebacterium sp. U-96 |
K358A | inactive | Corynebacterium sp. U-96 |
K358D | inactive | Corynebacterium sp. U-96 |
Y358R | the variant displays 0.07% activity and a higher apparent KM for sarcosine as compared to wild type enzyme | Corynebacterium sp. U-96 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + 5,6,7,8-tetrahydrofolate + O2 | Corynebacterium sp. U-96 | - |
glycine + formaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium sp. U-96 | Q50LF0 and Q50LF2 and Q50LE9 and Q50LF1 | subunits alpha, beta, gamma, and delta | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + 5,6,7,8-tetrahydrofolate + O2 | - |
Corynebacterium sp. U-96 | glycine + formaldehyde + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 1 * 103000 + 1 * 44000 + 1 * 21000 + 1 * 11000, SDS-PAGE | Corynebacterium sp. U-96 |
Synonyms | Comment | Organism |
---|---|---|
heterotetrameric sarcosine oxidase | - |
Corynebacterium sp. U-96 |
TSOX | - |
Corynebacterium sp. U-96 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Corynebacterium sp. U-96 | |
FMN | - |
Corynebacterium sp. U-96 | |
NAD+ | NAD+ is not involved in sarcosine oxidation or in redox equilibrium with the flavins. However, it has a critical effect on the folding and/or stability of the alpha subunit | Corynebacterium sp. U-96 |