Cloned (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
sarcosine | pH 8.5, 60°C | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, calculated, x * 51000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
51000 | - |
x * 43000, calculated, x * 51000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | Q6ITC6 | expression in Escherichia coli | - |
Bacillus sp. (in: Bacteria) BSD-8 | Q6ITC6 | expression in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
recombinant protein | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sarcosine + H2O + O2 | - |
Bacillus sp. (in: Bacteria) | glycine + formaldehyde + H2O2 | - |
? | |
sarcosine + H2O + O2 | - |
Bacillus sp. (in: Bacteria) BSD-8 | glycine + formaldehyde + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, calculated, x * 51000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable | Bacillus sp. (in: Bacteria) |
65 | - |
10 min, complete loss of activity | Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20.3 | - |
sarcosine | pH 8.5, 60°C | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | - |
Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 11 | - |
Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | wild-type enzyme, noncovalently bound, recombinant enzyme, covalently bound | Bacillus sp. (in: Bacteria) |