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Literature summary for 1.5.1.8 extracted from

  • Hutzler, J.; Dancis, J.
    Conversion of lysine to saccharopine by human tissues (1968), Biochim. Biophys. Acta, 158, 62-69.
    View publication on PubMed
Search for more literature for 1.5.1.8

Application

Application Comment Organism
medicine hyperlysinemia Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 1 mM, 71% inhibition Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH Homo sapiens lysine catabolism N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
9.6fold partial purification from liver Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland low enzyme concentration Homo sapiens
-
brain low enzyme concentration Homo sapiens
-
heart high enzyme concentration Homo sapiens
-
kidney after liver next highest enzyme concentration Homo sapiens
-
liver highest enzyme concentration Homo sapiens
-
additional information trace enzyme activity in lung, spleen, and striated muscle, no activity in pancreas, thymus, peripheral leucocyte or erythrocyte Homo sapiens
-
skin low enzyme concentration Homo sapiens
-
thyroid gland low enzyme concentration Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-25°C, 1 month, stable Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O epsilon-N-(L-glutaryl-2)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O epsilon-N-(L-glutaryl-2)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH lysine catabolism Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7 7
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.2 8.6 pH 6.2: about 50% of activity maximum, pH 8.6: about 60% of activity maximum Homo sapiens

Cofactor

Cofactor Comment Organism Structure
additional information NADH can not replace NADPH Homo sapiens
NADPH specific for NADPH Homo sapiens