Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3)-RIL cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E16Q/C205S | the mutation decreases the turnover number by about 15fold | Saccharomyces cerevisiae |
K13M/C205S | the mutation decreases the turnover number by about 15fold | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | Saccharomyces cerevisiae | - |
L-lysine + 2-oxoglutarate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA column chromatography | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
4°C, His-tagged mutant enzymes in 100 mM HEPES, 300 mM KCl and 300 mM imidazole at pH 8.0, several months, the mutant enzymes maintain stability and remain active | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | - |
Saccharomyces cerevisiae | L-lysine + 2-oxoglutarate + NADH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
N6-(glutaryl-2)-L-lysine: NAD+ oxidoreductase | - |
Saccharomyces cerevisiae |
SDH | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces cerevisiae |