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Literature summary for 1.5.1.6 extracted from

  • Champion, K.M.; Cook, R.J.; Tollaksen, S.L.; Giometti, C.S.
    Identification of a heritable deficiency of the folate-dependent enzyme 10-formyltetrahydrofolate dehydrogenase in mice (1994), Proc. Natl. Acad. Sci. USA, 91, 11338-11342.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information deletion mutation in the 10-formyltetrahydrofolate structural gene in homozygous mice with absence of FDH mRNA and enzyme activity, total folate pool is decreased and the level of tetrahydrofolate is markedly depleted, pairs of homozygotes have a dramatically decreased reproductive efficiency, may be attributable to fetal lethality Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Mus musculus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
96000
-
x * 96000, MSN13/94, identified as forms of FDH, two-dimensional electrophoresis Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O Mus musculus a major liver enzyme, contributing to more than one metabolic pathway, regeneration of the tetrahydrofolate pool, not essential for viability tetrahydrofolate + CO2 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
male mice, maintained on Teklad rodent diet no. 8604
-

Purification (Commentary)

Purification (Comment) Organism
partial purification of MSN13/94, identified as forms of 10-formyltetrahydrofolate dehydrogenase Mus musculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
no detectable activity in homozygous mice with deletion mutation in the FDH structural gene Mus musculus
0.00278
-
liver samples enriched for cytosolic proteins, heterozygous mice with deletion mutation in the FDH structural gene Mus musculus
0.00478
-
liver samples enriched for cytosolic proteins, normal mice Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O a major liver enzyme, contributing to more than one metabolic pathway, regeneration of the tetrahydrofolate pool, not essential for viability Mus musculus tetrahydrofolate + CO2 + NADPH + H+
-
?

Subunits

Subunits Comment Organism
? x * 96000, MSN13/94, identified as forms of FDH, two-dimensional electrophoresis Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate 10-formyltetrahydrofolate as coenzyme Mus musculus
tetrahydrofolate as coenzyme Mus musculus