Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion mutation in the 10-formyltetrahydrofolate structural gene in homozygous mice with absence of FDH mRNA and enzyme activity, total folate pool is decreased and the level of tetrahydrofolate is markedly depleted, pairs of homozygotes have a dramatically decreased reproductive efficiency, may be attributable to fetal lethality | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
96000 | - |
x * 96000, MSN13/94, identified as forms of FDH, two-dimensional electrophoresis | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | Mus musculus | a major liver enzyme, contributing to more than one metabolic pathway, regeneration of the tetrahydrofolate pool, not essential for viability | tetrahydrofolate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
male mice, maintained on Teklad rodent diet no. 8604 | - |
Purification (Comment) | Organism |
---|---|
partial purification of MSN13/94, identified as forms of 10-formyltetrahydrofolate dehydrogenase | Mus musculus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
no detectable activity in homozygous mice with deletion mutation in the FDH structural gene | Mus musculus |
0.00278 | - |
liver samples enriched for cytosolic proteins, heterozygous mice with deletion mutation in the FDH structural gene | Mus musculus |
0.00478 | - |
liver samples enriched for cytosolic proteins, normal mice | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | a major liver enzyme, contributing to more than one metabolic pathway, regeneration of the tetrahydrofolate pool, not essential for viability | Mus musculus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 96000, MSN13/94, identified as forms of FDH, two-dimensional electrophoresis | Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | 10-formyltetrahydrofolate as coenzyme | Mus musculus | |
tetrahydrofolate | as coenzyme | Mus musculus |