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Literature summary for 1.5.1.5 extracted from
- Purification and properties of N5, N10-Methylenetetra-hydrofolate dehydrogenase of calf thymus (1965), Biochim. Biophys. Acta, 105, 279-291.
General Stability
| General Stability | Organism |
|---|---|
| highly instable, partial protection against inactivation by thiol compounds, glycerol, serum albumin and EDTA, freezing inactivates purified enzyme | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Bos taurus |  |
| formaldehyde | above 0.4 mM | Bos taurus | |
| Mg2+ | - |
Bos taurus | |
| N-ethylmaleimide | - |
Bos taurus | |
| p-chloromercuribenzoate | inhibition reversible by dimercaptopropanol | Bos taurus | |
| phenacyl bromide | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
NADP+ | - |
Bos taurus | |
| 0.08 | - |
5,10-methylenetetrahydrofolate | - |
Bos taurus | |
| 0.17 | - |
5,10-methylenetetrahydrofolate | - |
Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | and other monovalent metal cations | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NADP+ | Bos taurus | - |
5,10-methenyltetrahydrofolate + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
young calves | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thymus | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -17°C, partially purified enzyme after second ammonium sulphate fractionation stable for 2 weeks, 1 mM mercaptoethanol, | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NADP+ | - |
Bos taurus | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
r | |
| 5,10-methylenetetrahydropteroyltriglutamate + NADP+ | - |
Bos taurus | 5,10-methenyltetrahydropteroyltriglutamate + NADPH | - |
? | |
| DL-tetrahydropteroyl-L-aspartate + NADP+ | - |
Bos taurus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 39.5 | - |
potassium phosphate buffer, pH 7.8 | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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