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Literature summary for 1.5.1.5 extracted from
- A 5,10-methylenetetrahydrofolate dehydrogenase: 5,10-methenyltetrahydrofolate cyclohydrolase protein from Pisum sativum (1995), Phytochemistry, 39, 1309-1317.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dihydropteroylmonoglutamate | competitive inhibition | Pisum sativum |  |
| dihydropteroylpentaglutamate | competitive inhibition | Pisum sativum | |
| pteroylglutamate | competitive inhibition | Pisum sativum | |
| pteroylglutamates | - |
Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
NADP+ | - |
Pisum sativum | |
| 0.021 | - |
tetrahydropteroylmonoglutamate | - |
Pisum sativum | |
| 0.025 | - |
Tetrahydropteroylpentaglutamate | - |
Pisum sativum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38500 | - |
? * 38500, probably not a heterodimer, SDS-PAGE | Pisum sativum |
| 58000 | - |
methylenetetrahydrofolate dehydrogenase-methyltetrahydrofolate cyclohydrolase protein, gel filtration | Pisum sativum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
L. cv. Homesteader | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| co-purification of 5,10-methylenetetrahydrofolate dehydrogenase and 5,10-methenyltetrahydrofolate cyclohydrolase | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Pisum sativum | - |
| leaf | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pisum sativum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 1 month, residual activity of 50% | Pisum sativum |
| 4°C, in the presence of 25% glycerol, 1 month, residual activity of 85% | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetrahydropteroylglutamic acid + NADP+ | - |
Pisum sativum | ? | - |
? | |
| tetrahydropteroylpentaglutamate + NADP+ | - |
Pisum sativum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | ? * 38500, probably not a heterodimer, SDS-PAGE | Pisum sativum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Pisum sativum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Pisum sativum | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
dihydropteroylpentaglutamate | varying concentration of tetrahydropteroylmonoglutamate | Pisum sativum | |
| 0.035 | - |
dihydropteroylmonoglutamate | varying concentration of tetrahydropteroylmonoglutamate | Pisum sativum | |
| 0.61 | - |
pteroylglutamate | varying concentration of tetrahydropteroylmonoglutamate | Pisum sativum | |
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