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Literature summary for 1.5.1.5 extracted from

  • Tan, L.U.L.; Drury, E.J.; MacKenzie, R.E.
    Methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase. A multifunctional protein from porcine liver (1977), J. Biol. Chem., 252, 1117-1122.
    View publication on PubMed
Search for more literature for 1.5.1.5

General Stability

General Stability Organism
stable for 1 week, stored in 20% glycerol, 0.1 M potassium phosphate, pH 7.3 Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
folic acid competitive against methylenetetrahydrofolate Sus scrofa
NAD+
-
 Sus scrofa
NADPH
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 NADP+
-
 Sus scrofa
0.022
-
 5,10-methylenetetrahydrofolate
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 1 * 100000, methylenetetrahydrofolate dehydrogenase, formyltetrahydrofolate synthetase and methenyltetrahydrofolate cyclohydrolase combined, SDS-PAGE Sus scrofa
150000
-
 methylenetetrahydrofolate dehydrogenase, formyltetrahydrofolate synthetase and methenyltetrahydrofolate cyclohydrolase combined, gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methylenetetrahydrofolate + NADP+ Sus scrofa
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
trifunctional enzyme: methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrase, formyltetrahydrofolate synthetase Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADP+
-
 Sus scrofa 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000, methylenetetrahydrofolate dehydrogenase, formyltetrahydrofolate synthetase and methenyltetrahydrofolate cyclohydrolase combined, SDS-PAGE Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Sus scrofa