Cloned (Comment) | Organism |
---|---|
gene dmrA, phylogenetic analysis and tree, recombinant expression of N-terminally His6-tagged and C-terminally His4-tagged enzyme in Escherichia coli strain BL21 | Methylorubrum extorquens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Methylorubrum extorquens | |
0.102 | - |
dihydrosarcinapterin | pH 6.8, 23°C | Hyphomicrobium nitrativorans | |
0.193 | - |
7,8-dihydromethanopterin | pH 5.3, 23°C, recombinant C-terminally His4-tagged enzyme | Methylorubrum extorquens | |
0.195 | - |
dihydrosarcinapterin | pH 5.3, temperature not specified in the publication | Methylorubrum extorquens | |
0.695 | - |
dihydrosarcinapterin | pH 6.8, 23°C | Methylobacterium nodulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydromethanopterin + NADPH + H+ | Methylorubrum extorquens | - |
5,6,7,8-tetrahydromethanopterin + NADP+ | - |
r | |
7,8-dihydromethanopterin + NADPH + H+ | Methylorubrum extorquens NCIMB 9133 | - |
5,6,7,8-tetrahydromethanopterin + NADP+ | - |
r | |
7,8-dihydromethanopterin + NADPH + H+ | Methylorubrum extorquens DSM 1338 | - |
5,6,7,8-tetrahydromethanopterin + NADP+ | - |
r | |
7,8-dihydromethanopterin + NADPH + H+ | Methylorubrum extorquens ATCC 14718 | - |
5,6,7,8-tetrahydromethanopterin + NADP+ | - |
r | |
7,8-dihydromethanopterin + NADPH + H+ | Methylorubrum extorquens JCM 2805 | - |
5,6,7,8-tetrahydromethanopterin + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyphomicrobium nitrativorans | - |
- |
- |
Methylobacterium nodulans | B8IHA6 | - |
- |
Methylobacterium nodulans LMG 21967 | B8IHA6 | - |
- |
Methylorubrum extorquens | C5B2R8 | - |
- |
Methylorubrum extorquens | C5B2R8 | Methylobacterium extorquens | - |
Methylorubrum extorquens ATCC 14718 | C5B2R8 | Methylobacterium extorquens | - |
Methylorubrum extorquens DSM 1338 | C5B2R8 | - |
- |
Methylorubrum extorquens DSM 1338 | C5B2R8 | Methylobacterium extorquens | - |
Methylorubrum extorquens JCM 2805 | C5B2R8 | Methylobacterium extorquens | - |
Methylorubrum extorquens NCIMB 9133 | C5B2R8 | Methylobacterium extorquens | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzymes from Escherichia coli strain BL21 by nickel affinity chromatography | Methylorubrum extorquens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the facultative methylotroph Methylobacterium extorquens AM1, growth on single-carbon (C1) substrates involves the use of both tetrahydromethanopterin (H4MPT) and tetrahydrofolate (H4F) | Methylorubrum extorquens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.63 | - |
substrate dihydrosarcinapterin, pH 6.8, 23°C | Methylobacterium nodulans |
2.24 | - |
substrate dihydrosarcinapterin, pH 5.3, temperature not specified in the publication | Methylorubrum extorquens |
2.82 | - |
substrate dihydrosarcinapterin, pH 6.8, 23°C | Hyphomicrobium nitrativorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydromethanopterin + NADPH + H+ | - |
Methylorubrum extorquens | 5,6,7,8-tetrahydromethanopterin + NADP+ | - |
? | |
7,8-dihydromethanopterin + NADPH + H+ | - |
Methylorubrum extorquens | 5,6,7,8-tetrahydromethanopterin + NADP+ | - |
r | |
7,8-dihydromethanopterin + NADPH + H+ | - |
Methylobacterium nodulans | 5,6,7,8-tetrahydromethanopterin + NADP+ | - |
? | |
7,8-dihydromethanopterin + NADPH + H+ | - |
Hyphomicrobium nitrativorans | 5,6,7,8-tetrahydromethanopterin + NADP+ | - |
? | |
7,8-dihydromethanopterin + NADPH + H+ | - |
Methylorubrum extorquens NCIMB 9133 | 5,6,7,8-tetrahydromethanopterin + NADP+ | - |
? | |
7,8-dihydromethanopterin + NADPH + H+ | - |
Methylorubrum extorquens NCIMB 9133 | 5,6,7,8-tetrahydromethanopterin + NADP+ | - |
r | |
7,8-dihydromethanopterin + NADPH + H+ | - |
Methylorubrum extorquens DSM 1338 | 5,6,7,8-tetrahydromethanopterin + NADP+ | - |
? | |
7,8-dihydromethanopterin + NADPH + H+ | - |
Methylorubrum extorquens DSM 1338 | 5,6,7,8-tetrahydromethanopterin + NADP+ | - |
r | |
7,8-dihydromethanopterin + NADPH + H+ | - |
Methylorubrum extorquens ATCC 14718 | 5,6,7,8-tetrahydromethanopterin + NADP+ | - |
? | |
7,8-dihydromethanopterin + NADPH + H+ | - |
Methylorubrum extorquens ATCC 14718 | 5,6,7,8-tetrahydromethanopterin + NADP+ | - |
r | |
7,8-dihydromethanopterin + NADPH + H+ | - |
Methylobacterium nodulans LMG 21967 | 5,6,7,8-tetrahydromethanopterin + NADP+ | - |
? | |
7,8-dihydromethanopterin + NADPH + H+ | - |
Methylorubrum extorquens JCM 2805 | 5,6,7,8-tetrahydromethanopterin + NADP+ | - |
? | |
7,8-dihydromethanopterin + NADPH + H+ | - |
Methylorubrum extorquens JCM 2805 | 5,6,7,8-tetrahydromethanopterin + NADP+ | - |
r | |
dihydrosarcinapterin + NADPH + H+ | - |
Methylorubrum extorquens | tetrahydrosarcinapterin + NADP+ | - |
? | |
dihydrosarcinapterin + NADPH + H+ | - |
Methylobacterium nodulans | tetrahydrosarcinapterin + NADP+ | - |
? | |
dihydrosarcinapterin + NADPH + H+ | - |
Hyphomicrobium nitrativorans | tetrahydrosarcinapterin + NADP+ | - |
? | |
dihydrosarcinapterin + NADPH + H+ | - |
Methylorubrum extorquens NCIMB 9133 | tetrahydrosarcinapterin + NADP+ | - |
? | |
dihydrosarcinapterin + NADPH + H+ | - |
Methylorubrum extorquens DSM 1338 | tetrahydrosarcinapterin + NADP+ | - |
? | |
dihydrosarcinapterin + NADPH + H+ | - |
Methylorubrum extorquens ATCC 14718 | tetrahydrosarcinapterin + NADP+ | - |
? | |
dihydrosarcinapterin + NADPH + H+ | - |
Methylobacterium nodulans LMG 21967 | tetrahydrosarcinapterin + NADP+ | - |
? | |
dihydrosarcinapterin + NADPH + H+ | - |
Methylorubrum extorquens JCM 2805 | tetrahydrosarcinapterin + NADP+ | - |
? | |
additional information | no activity with 7,8-dihydrofolate | Methylobacterium nodulans | ? | - |
- |
|
additional information | no activity with 7,8-dihydrofolate | Hyphomicrobium nitrativorans | ? | - |
- |
|
additional information | no activity with 7,8-dihydrofolate | Methylobacterium nodulans LMG 21967 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 15800, C-terminally His4-tagged enzyme DmrA, SDS-PAGE | Methylorubrum extorquens |
Synonyms | Comment | Organism |
---|---|---|
dihydromethanopterin reductase | - |
Methylorubrum extorquens |
DmrA | - |
Methylorubrum extorquens |
Mnod_4941 | - |
Methylobacterium nodulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 37 | broad | Methylobacterium nodulans |
15 | 37 | broad | Hyphomicrobium nitrativorans |
23 | - |
assay at room temperature | Methylorubrum extorquens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
7,8-dihydromethanopterin | pH 5.3, 23°C, recombinant C-terminally His4-tagged enzyme | Methylorubrum extorquens | |
1.5 | - |
dihydrosarcinapterin | pH 5.3, temperature not specified in the publication | Methylorubrum extorquens | |
1.6 | - |
dihydrosarcinapterin | pH 6.8, 23°C | Methylobacterium nodulans | |
2.9 | - |
dihydrosarcinapterin | pH 6.8, 23°C | Hyphomicrobium nitrativorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
assay at | Methylorubrum extorquens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Methylorubrum extorquens |
General Information | Comment | Organism |
---|---|---|
evolution | homology of DmrA to dihydrofolate reductases leads to the proposal that DmrA evolved from an ancestral dihydrofolate reductase following horizontal transfer of tetrahydromethanopterin (H4MPT) biosynthesis genes from anaerobic archaea to aerobic bacteria. Methylobacterium extorquens AM1 contains one dihydromethanopterin reductase (DmrA) and two putative dihydrofolate reductases, DfrA and DfrB (EC 1.5.1.3), that, respectively, share 26% identity (41% similarity) and 34% identity (53% similarity) with DmrA. DmrA shares no sequence homology with the FMN-containing dihydromethanopterin reductase discovered in archaea (DmrX) or related archaeallike flavoproteins (AfpA and DmrB) from beta-proteobacteria. Phylogenetic analysis and tree. Conformational modeling of DmrA and DfrB | Methylorubrum extorquens |
metabolism | dihydromethanopterin reductase (DmrA) catalyzes the final step of tetrahydromethanopterin (H4MPT) biosynthesis. In the pathways of H4MPT and tetrahydrofolate (H4F) biosynthesis, the last step requires the activity of dihydromethanopterin reductase (Dmr) or dihydrofolate reductase (Dfr). Methylobacterium extorquens AM1 contains one dihydromethanopterin reductase (DmrA) and two putative dihydrofolate reductases, DfrA and DfrB, that, respectively, share 26% identity (41% similarity) and 34% identity (53% similarity) with DmrA | Methylorubrum extorquens |
physiological function | methane-producing archaea and methylotrophic bacteria use tetrahydromethanopterin (H4MPT) and/or tetrahydrofolate (H4F) as coenzymes in one-carbon (C1) transfer pathways. Dihydromethanopterin reductase (DmrA) catalyzes the final step of tetrahydromethanopterin (H4MPT) biosynthesis. The facultative methylotroph Methylobacterium extorquens AM1, growth on single-carbon (C1) substrates involves the use of both tetrahydromethanopterin (H4MPT) and tetrahydrofolate (H4F) | Methylorubrum extorquens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.77 | - |
7,8-dihydromethanopterin | pH 5.3, 23°C, recombinant C-terminally His4-tagged enzyme | Methylorubrum extorquens |