Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL1 Blue MRF cells | Aspergillus fumigatus |
expressed in Escherichia coli XL1 Blue MRF cells | Penicillium commune |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
1 * 30000, His6-tagged enzyme, SDS-PAGe | Aspergillus fumigatus |
30000 | - |
1 * 30000, His6-tagged enzyme, SDS-PAGe | Penicillium commune |
32600 | - |
1 * 32600, His6-tagged enzyme, calculated from amino acid sequence | Aspergillus fumigatus |
32600 | - |
1 * 32600, His6-tagged enzyme, calculated from amino acid sequence | Penicillium commune |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | - |
- |
- |
Aspergillus fumigatus Af293 | - |
- |
- |
Claviceps purpurea | - |
- |
- |
Penicillium commune | I6U936 | - |
- |
Penicillium commune NRRL2033 | I6U936 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and HisPur Cobalt resin column chromatography | Aspergillus fumigatus |
Ni-NTA agarose column chromatography and HisPur Cobalt resin column chromatography | Penicillium commune |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chanoclavine-I aldehyde + NADH + H+ | festuclavine synthase FgaFS and the old yellow enzyme FgaOx3 from Aspergillus fumigatus catalyse the formation of festuclavine by two reduction steps | Aspergillus fumigatus | festuclavine + NAD+ | - |
? | |
chanoclavine-I aldehyde + NADH + H+ | festuclavine synthase FgaFS and the old yellow enzyme FgaOx3 from Aspergillus fumigatus catalyse the formation of festuclavine by two reduction steps | Penicillium commune | festuclavine + NAD+ | - |
? | |
chanoclavine-I aldehyde + NADH + H+ | festuclavine synthase FgaFS and the old yellow enzyme FgaOx3 from Aspergillus fumigatus catalyse the formation of festuclavine by two reduction steps | Aspergillus fumigatus Af293 | festuclavine + NAD+ | - |
? | |
chanoclavine-I aldehyde + NADH + H+ | festuclavine synthase FgaFS and the old yellow enzyme FgaOx3 from Aspergillus fumigatus catalyse the formation of festuclavine by two reduction steps | Penicillium commune NRRL2033 | festuclavine + NAD+ | - |
? | |
additional information | in the absence of reduced glutathione, agroclavine synthase EasG also converts chanoclavine-I aldehyde to pyroclavine and festuclavine in the presence of the old yellow enzyme FgaOx3 and NADPH | Claviceps purpurea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, His6-tagged enzyme, SDS-PAGe | Aspergillus fumigatus |
monomer | 1 * 30000, His6-tagged enzyme, SDS-PAGe | Penicillium commune |
monomer | 1 * 32600, His6-tagged enzyme, calculated from amino acid sequence | Aspergillus fumigatus |
monomer | 1 * 32600, His6-tagged enzyme, calculated from amino acid sequence | Penicillium commune |
Synonyms | Comment | Organism |
---|---|---|
EasG | - |
Claviceps purpurea |
festuclavine synthase | - |
Aspergillus fumigatus |
festuclavine synthase | - |
Penicillium commune |
FgaFS | - |
Aspergillus fumigatus |
FgaFS | - |
Penicillium commune |