Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.44 extracted from

  • Matuschek, M.; Wallwey, C.; Wollinsky, B.; Xie, X.; Li, S.
    In vitro conversion of chanoclavine-I aldehyde to the stereoisomers festuclavine and pyroclavine controlled by the second reduction step (2012), RSC Adv., 2, 3662-3669.
No PubMed abstract available
Search for more literature for 1.5.1.44

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL1 Blue MRF cells Aspergillus fumigatus
expressed in Escherichia coli XL1 Blue MRF cells Penicillium commune

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 1 * 30000, His6-tagged enzyme, SDS-PAGe Aspergillus fumigatus
30000
-
 1 * 30000, His6-tagged enzyme, SDS-PAGe Penicillium commune
32600
-
 1 * 32600, His6-tagged enzyme, calculated from amino acid sequence Aspergillus fumigatus
32600
-
 1 * 32600, His6-tagged enzyme, calculated from amino acid sequence Penicillium commune

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-
Aspergillus fumigatus Af293
-
-
-
Claviceps purpurea
-
-
-
Penicillium commune I6U936
-
-
Penicillium commune NRRL2033 I6U936
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and HisPur Cobalt resin column chromatography Aspergillus fumigatus
Ni-NTA agarose column chromatography and HisPur Cobalt resin column chromatography Penicillium commune

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chanoclavine-I aldehyde + NADH + H+ festuclavine synthase FgaFS and the old yellow enzyme FgaOx3 from Aspergillus fumigatus catalyse the formation of festuclavine by two reduction steps Aspergillus fumigatus festuclavine + NAD+
-
 ?
chanoclavine-I aldehyde + NADH + H+ festuclavine synthase FgaFS and the old yellow enzyme FgaOx3 from Aspergillus fumigatus catalyse the formation of festuclavine by two reduction steps Penicillium commune festuclavine + NAD+
-
 ?
chanoclavine-I aldehyde + NADH + H+ festuclavine synthase FgaFS and the old yellow enzyme FgaOx3 from Aspergillus fumigatus catalyse the formation of festuclavine by two reduction steps Aspergillus fumigatus Af293 festuclavine + NAD+
-
 ?
chanoclavine-I aldehyde + NADH + H+ festuclavine synthase FgaFS and the old yellow enzyme FgaOx3 from Aspergillus fumigatus catalyse the formation of festuclavine by two reduction steps Penicillium commune NRRL2033 festuclavine + NAD+
-
 ?
additional information in the absence of reduced glutathione, agroclavine synthase EasG also converts chanoclavine-I aldehyde to pyroclavine and festuclavine in the presence of the old yellow enzyme FgaOx3 and NADPH Claviceps purpurea ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30000, His6-tagged enzyme, SDS-PAGe Aspergillus fumigatus
monomer 1 * 30000, His6-tagged enzyme, SDS-PAGe Penicillium commune
monomer 1 * 32600, His6-tagged enzyme, calculated from amino acid sequence Aspergillus fumigatus
monomer 1 * 32600, His6-tagged enzyme, calculated from amino acid sequence Penicillium commune

Synonyms

Synonyms Comment Organism
EasG
-
 Claviceps purpurea
festuclavine synthase
-
 Aspergillus fumigatus
festuclavine synthase
-
 Penicillium commune
FgaFS
-
 Aspergillus fumigatus
FgaFS
-
 Penicillium commune