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Literature summary for 1.5.1.43 extracted from

  • Nakao, H.; Shinoda, S.; Yamamoto, S.
    Purification and some properties of carboxynorspermidine synthase participating in a novel biosynthetic pathway for norspermidine inVibrio alginolyticus (1991), J. Gen. Microbiol., 137, 1737-1742.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol about 14fold increase of activity at 20 mM. Without addition of dithiothreitol to the assay mixture, only 7% of the maximum activity is detected in the purified enzyme Vibrio alginolyticus
additional information EDTA (1 mM) shows no significant stimulatory effect on enzyme activity Vibrio alginolyticus

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide 24% inhibition at 5 mM Vibrio alginolyticus
additional information EDTA (1 mM) shows no significant inhibitory effect on enzyme activity; NAD+, ATP and SPD are not inhibitory at 5 mM Vibrio alginolyticus
N-ethylmaleimide 83% inhibition at 5 mM Vibrio alginolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.51
-
NADPH at pH 7.5 and 37°C Vibrio alginolyticus
2.97
-
NADH at pH 7.5 and 37°C Vibrio alginolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Ca2+, Mg2+, Fe3+, Fe2+, Cu+, Cu2+, Mn2+, Zn2+, Na+ and K+ at 1 M have no effect on enzyme activity Vibrio alginolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45100
-
2 * 45100, SDS-PAGE Vibrio alginolyticus
93500
-
gel filtration Vibrio alginolyticus

Organism

Organism UniProt Comment Textmining
Vibrio alginolyticus
-
-
-
Vibrio alginolyticus ATCC 17749
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Blue Sepharose CL-6B column chromatography, DEAE-Sepharose column chromatography, and hydroxyapatite column chromatography Vibrio alginolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0167
-
crude enzyme, at pH 7.5 and 37°C Vibrio alginolyticus
31
-
after 1856fold purification, at pH 7.5 and 37°C Vibrio alginolyticus

Storage Stability

Storage Stability Organism
4°C, in 20 mM Tris/HCl, pH 7.5, containing 1 mM dithiothreitol and 0.02% (v/v) NaN3, 1 week, 15% loss of activity Vibrio alginolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADH + H+ NADH is a much poorer cofactor than NADPH Vibrio alginolyticus carboxynorspermidine + H2O + NAD+
-
?
L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
Vibrio alginolyticus carboxynorspermidine + H2O + NADP+
-
?
L-aspartate 4-semialdehyde + putrescine + NADPH + H+ 7% activity with putrescine compared to propane-1,3-diamine Vibrio alginolyticus carboxyspermidine + H2O + NADP+
-
?
additional information does not react with ethylenediamine, cadaverine and D-aspartate 4-semialdehyde Vibrio alginolyticus ?
-
?
additional information does not react with ethylenediamine, cadaverine and D-aspartate 4-semialdehyde Vibrio alginolyticus ATCC 17749 ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 45100, SDS-PAGE Vibrio alginolyticus

Synonyms

Synonyms Comment Organism
ASA-DAP Schiff base reductase
-
Vibrio alginolyticus
C-NSPD synthase
-
Vibrio alginolyticus
carboxynorspermidine synthase
-
Vibrio alginolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Vibrio alginolyticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 78% and 59% of the maximum activity remain at 30°C and 45°C, respectively, but no activity is observed above 50°C Vibrio alginolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 7.5
-
Vibrio alginolyticus

pH Range

pH Minimum pH Maximum Comment Organism
6.3 8 85% and 18% of the maximum activity are observed at pH 8.0 and 6.3, respectively Vibrio alginolyticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
the enzyme is unstable in buffers of pH below 6.5. At pH 6.5, 50% loss of activity is observed within 12 h at 4°C Vibrio alginolyticus

Cofactor

Cofactor Comment Organism Structure
NADH NADH is a much poorer cofactor than NADPH Vibrio alginolyticus
NADPH
-
Vibrio alginolyticus

pI Value

Organism Comment pI Value Maximum pI Value
Vibrio alginolyticus isoelectric focusing 4.3 4.2

Expression

Organism Comment Expression
Vibrio alginolyticus when Vibrio alginolyticus is grown in the presence of 5 mM norspermidine, the specific activity of the enzyme is reduced by about 70% down