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Literature summary for 1.5.1.40 extracted from

  • Yamazaki, S.; Tsai, L.
    Purification and properties of 8-hydroxy-5-deazaflavin-dependent NADP+ reductase from Methanococcus vannielii (1980), J. Biol. Chem., 255, 6462-6465.
    View publication on PubMed
Search for more literature for 1.5.1.40

Inhibitors

Inhibitors Comment Organism Structure
4-Chloromercuriphenyl sulfonate
-
 Methanococcus vannielii
iodoacetamide
-
 Methanococcus vannielii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 1,5-dihydro-8-hydroxy-5-deazaflavin pH 7.0, 20°C Methanococcus vannielii
0.008
-
 8-hydroxy-5-deazaflavin pH 7.0, 20°C Methanococcus vannielii
0.0104
-
 NADPH pH 7.0, 20°C Methanococcus vannielii
0.0137
-
 NADP+ pH 7.0, 20°C Methanococcus vannielii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 2 * 43000, SDS-PAGE Methanococcus vannielii
85000
-
 gel filtration Methanococcus vannielii

Organism

Organism UniProt Comment Textmining
Methanococcus vannielii
-
-
-
Methanococcus vannielii DSM 1224
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanococcus vannielii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.79
-
 pH 7.0, 20°C Methanococcus vannielii

Storage Stability

Storage Stability Organism
-75°C, 2 months, at low concentration (0.066 mg/ml), in the presence of ethylene glycol, 20% (v/v), no loss of activity Methanococcus vannielii
4°C, at low concentration (0.066 mg/ml), in the presence of ethylene glycol, 20% (v/v), 22% of the activity is lost Methanococcus vannielii
4°C, at low concentration (0.066 mg/ml), the purified enzyme loses 84% of its activity in 2 days Methanococcus vannielii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-dihydro-8-hydroxy-5-deazaflavin + NADP+ the kcat value of the forward reaction is 24 times greater than that of the reverse reaction, thus the production of NADPH at pH 7.0 is more favorable than its consumption Methanococcus vannielii 8-hydroxy-5-deazaflavin + NADPH + H+
-
 r
1,5-dihydro-8-hydroxy-5-deazaflavin + NADP+ the kcat value of the forward reaction is 24 times greater than that of the reverse reaction, thus the production of NADPH at pH 7.0 is more favorable than its consumption Methanococcus vannielii DSM 1224 8-hydroxy-5-deazaflavin + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 43000, SDS-PAGE Methanococcus vannielii

Synonyms

Synonyms Comment Organism
8-hydroxy-5-deazaflavin-dependent NADP+ reductase the enzyme is a component of the formate NADP+ oxidoreductase system of Methanococcus vannielii Methanococcus vannielii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
17 20
-
 Methanococcus vannielii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.37
-
 NADP+ pH 7.0, 20°C Methanococcus vannielii
7.37
-
 1,5-dihydro-8-hydroxy-5-deazaflavin pH 7.0, 20°C Methanococcus vannielii
175
-
 NADPH pH 7.0, 20°C Methanococcus vannielii
175
-
 8-hydroxy-5-deazaflavin pH 7.0, 20°C Methanococcus vannielii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
 reaction with 8-hydroxy-5-deazaflavin + NADPH Methanococcus vannielii
7.9
-
 reaction with 1,5-dihydro-8-hydroxyy-5-deazaflavin + NADP+ Methanococcus vannielii