Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
FMN | when NADPH is supplied as pyrimidinic substrate, maximal reductase activity is obtained with 2.5-10 mM FMN, while higher FMN concentration led to 15% decrease in SsuE activity. When NADH is the pyrimidinic substrate, a distinct activity maximum is obtained at an FMN concentration of 0.5 mM, whereas concentrations higher than 2.5 mM led to more than 60% decrease in specific activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000054 | - |
FMN | pH 7.9, 30°C | Escherichia coli | |
0.0027 | - |
FAD | pH 7.9, 30°C | Escherichia coli | |
0.046 | - |
NADPH | pH 7.9, 30°C, 0.003 mM FMN | Escherichia coli | |
0.5555 | - |
NADH | pH 7.9, 30°C, 0.0005 mM FMN | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23700 | - |
2 * 23700, calculated from sequence | Escherichia coli |
25400 | - |
2 * 25400, SDS-PAGE | Escherichia coli |
58400 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P80644 | - |
- |
Purification (Comment) | Organism |
---|---|
SsuE is purified to homogeneity as an N-terminal histidine-tagged fusion protein | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FAD + NADPH + H+ | FMN is the preferred flavin substrate of SsuE but FAD and riboflavin are also reduced at significant rates, whereas lumiflavin is not | Escherichia coli | FADH2 + NADP+ | - |
? | |
FMN + NADH + H+ | when NADH is the pyrimidinic substrate, a distinct activity maximum is obtained at an FMN concentration of 0.5 mM, whereas concentrations higher than 2.5 mM led to more than 60% decrease in specific activity | Escherichia coli | FMNH2 + NAD+ | - |
? | |
FMN + NADPH + H+ | FMN is the preferred flavin substrate of SsuE but FAD and riboflavin are also reduced at significant rates, whereas lumiflavin is not. When NADPH is supplied as pyrimidinic substrate, maximal reductase activity is obtained with 2.5-10 mM FMN, while higher FMN concentration leads to 15% decrease in SsuE activity. When NADH is the pyrimidinic substrate, a distinct activity maximum is obtained at an FMN concentration of 0.5 mM, whereas concentrations higher than 2.5 mM led to more than 60% decrease in specific activity | Escherichia coli | FMNH2 + NADP+ | - |
? | |
riboflavin + NADPH + H+ | FMN is the preferred flavin substrate of SsuE but FAD and riboflavin are also reduced at significant rates, whereas lumiflavin is not | Escherichia coli | reduced riboflavin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 23700, calculated from sequence | Escherichia coli |
homodimer | 2 * 25400, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
SsuE | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not contain any bound flavin cofactor | Escherichia coli | |
NADH | kcat/KM for NADPH is 335fold higher compared to kcat/KM for NADH | Escherichia coli | |
NADPH | kcat/KM for NADPH is 335fold higher compared kcat/KM for NADH | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | SsuD is a monooxygenase that catalyzes the desulfonation of alkanesulfonates and requires reduced FMN, which is provided by the NAD(P)H:flavin oxidoreductase SsuE | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00167 | - |
NADH | pH 7.9, 30°C, 0.0005 mM FMN | Escherichia coli | |
0.0079 | - |
FMN | pH 7.9, 30°C | Escherichia coli | |
0.28 | - |
FAD | pH 7.9, 30°C | Escherichia coli | |
0.56 | - |
NADPH | pH 7.9, 30°C, 0.003 mM FMN | Escherichia coli |