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Literature summary for 1.5.1.38 extracted from

  • Eichhorn, E.; van der Ploeg, J.R.; Leisinger, T.
    Characterization of a two-component alkanesulfonate monooxygenase from Escherichia coli (1999), J. Biol. Chem., 274, 26639-26646.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
FMN when NADPH is supplied as pyrimidinic substrate, maximal reductase activity is obtained with 2.5-10 mM FMN, while higher FMN concentration led to 15% decrease in SsuE activity. When NADH is the pyrimidinic substrate, a distinct activity maximum is obtained at an FMN concentration of 0.5 mM, whereas concentrations higher than 2.5 mM led to more than 60% decrease in specific activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000054
-
FMN pH 7.9, 30°C Escherichia coli
0.0027
-
FAD pH 7.9, 30°C Escherichia coli
0.046
-
NADPH pH 7.9, 30°C, 0.003 mM FMN Escherichia coli
0.5555
-
NADH pH 7.9, 30°C, 0.0005 mM FMN Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23700
-
2 * 23700, calculated from sequence Escherichia coli
25400
-
2 * 25400, SDS-PAGE Escherichia coli
58400
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P80644
-
-

Purification (Commentary)

Purification (Comment) Organism
SsuE is purified to homogeneity as an N-terminal histidine-tagged fusion protein Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FAD + NADPH + H+ FMN is the preferred flavin substrate of SsuE but FAD and riboflavin are also reduced at significant rates, whereas lumiflavin is not Escherichia coli FADH2 + NADP+
-
?
FMN + NADH + H+ when NADH is the pyrimidinic substrate, a distinct activity maximum is obtained at an FMN concentration of 0.5 mM, whereas concentrations higher than 2.5 mM led to more than 60% decrease in specific activity Escherichia coli FMNH2 + NAD+
-
?
FMN + NADPH + H+ FMN is the preferred flavin substrate of SsuE but FAD and riboflavin are also reduced at significant rates, whereas lumiflavin is not. When NADPH is supplied as pyrimidinic substrate, maximal reductase activity is obtained with 2.5-10 mM FMN, while higher FMN concentration leads to 15% decrease in SsuE activity. When NADH is the pyrimidinic substrate, a distinct activity maximum is obtained at an FMN concentration of 0.5 mM, whereas concentrations higher than 2.5 mM led to more than 60% decrease in specific activity Escherichia coli FMNH2 + NADP+
-
?
riboflavin + NADPH + H+ FMN is the preferred flavin substrate of SsuE but FAD and riboflavin are also reduced at significant rates, whereas lumiflavin is not Escherichia coli reduced riboflavin + NADP+
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 23700, calculated from sequence Escherichia coli
homodimer 2 * 25400, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
SsuE
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme does not contain any bound flavin cofactor Escherichia coli
NADH kcat/KM for NADPH is 335fold higher compared to kcat/KM for NADH Escherichia coli
NADPH kcat/KM for NADPH is 335fold higher compared kcat/KM for NADH Escherichia coli

General Information

General Information Comment Organism
physiological function SsuD is a monooxygenase that catalyzes the desulfonation of alkanesulfonates and requires reduced FMN, which is provided by the NAD(P)H:flavin oxidoreductase SsuE Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00167
-
NADH pH 7.9, 30°C, 0.0005 mM FMN Escherichia coli
0.0079
-
FMN pH 7.9, 30°C Escherichia coli
0.28
-
FAD pH 7.9, 30°C Escherichia coli
0.56
-
NADPH pH 7.9, 30°C, 0.003 mM FMN Escherichia coli