Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a C-terminally His-tagged fusion protein | Paraburkholderia phenoliruptrix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0048 | - |
FAD | cosubstrate: NADH, pH 7.0, 24°C | Paraburkholderia phenoliruptrix | |
0.0103 | - |
FMN | cosubstrate: NADH, pH 7.0, 24°C | Paraburkholderia phenoliruptrix | |
0.0401 | - |
NADH | cosubstrate: FAD, pH 7.0, 24°C | Paraburkholderia phenoliruptrix | |
0.164 | - |
NADH | cosubstrate: FMN, pH 7.0, 24°C | Paraburkholderia phenoliruptrix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
x * 22000, His-tagged TftC, SDS-PAGE | Paraburkholderia phenoliruptrix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paraburkholderia phenoliruptrix | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Paraburkholderia phenoliruptrix |
Storage Stability | Organism |
---|---|
-80°C, His-tagged TftC loses about 50% activity after 2 months | Paraburkholderia phenoliruptrix |
0°C, His-tagged TftC is unstable in the elution buffer containing 140 mM imidazole on ice, losing about 50% activity in 2 h | Paraburkholderia phenoliruptrix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FAD + NADH + H+ | FAD is a better substrate than FMN. The enzyme does not use NADPH or riboflavin as substrate | Paraburkholderia phenoliruptrix | FADH2 + NAD+ | - |
? | |
FMN + NADH + H+ | FAD is a better substrate than FMN. The enzyme does not use NADPH or riboflavin as substrate | Paraburkholderia phenoliruptrix | FMNH2 + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 22000, His-tagged TftC, SDS-PAGE | Paraburkholderia phenoliruptrix |
Synonyms | Comment | Organism |
---|---|---|
NADH-dependent FAD reductase | - |
Paraburkholderia phenoliruptrix |
NADH-FAD reductase | - |
Paraburkholderia phenoliruptrix |
NADH:FAD oxidoreductase | - |
Paraburkholderia phenoliruptrix |
NADH:flavin adenine dinucleotide oxidoreductase | - |
Paraburkholderia phenoliruptrix |
TftC | - |
Paraburkholderia phenoliruptrix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
assay at | Paraburkholderia phenoliruptrix |
30 | - |
- |
Paraburkholderia phenoliruptrix |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | 45 | 23°C: 85% of maximal activity, 45°C: 70% of maximal activity | Paraburkholderia phenoliruptrix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.9 | - |
NADH | cosubstrate: FMN, pH 7.0, 24°C | Paraburkholderia phenoliruptrix | |
16.6 | - |
FAD | cosubstrate: NADH, pH 7.0, 24°C | Paraburkholderia phenoliruptrix | |
18.5 | - |
FMN | cosubstrate: NADH, pH 7.0, 24°C | Paraburkholderia phenoliruptrix | |
30.1 | - |
NADH | cosubstrate: FAD, pH 7.0, 24°C | Paraburkholderia phenoliruptrix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
Na-phosphate buffer | Paraburkholderia phenoliruptrix |
7 | - |
assay at | Paraburkholderia phenoliruptrix |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.6 | pH 5.0 (sodium succinate buffer): 52% of maximal activity, pH 7.6: 75% of maximal activity | Paraburkholderia phenoliruptrix |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | the enzyme does not use NADPH as substrate | Paraburkholderia phenoliruptrix |
General Information | Comment | Organism |
---|---|---|
physiological function | TftD supplies FADH2 to (FADH2)-utilizing monooxygenase TftD, that converts 2,4,5-trichlorophenol to 2,5-dichloro-p-quinol and then to 5-chlorohydroxyquinol. It also converts 2,4,6-trichlorophenol to 2,6-dichloro-p-quinol as the final product | Paraburkholderia phenoliruptrix |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
72.5 | - |
NADH | cosubstrate: FMN, pH 7.0, 24°C | Paraburkholderia phenoliruptrix | |
77.3 | - |
NADH | cosubstrate: FAD, pH 7.0, 24°C | Paraburkholderia phenoliruptrix | |
1796 | - |
FMN | cosubstrate: NADH, pH 7.0, 24°C | Paraburkholderia phenoliruptrix | |
3458 | - |
FAD | cosubstrate: NADH, pH 7.0, 24°C | Paraburkholderia phenoliruptrix |