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Literature summary for 1.5.1.34 extracted from
- Dihydropteridine reductase as an alternative to dihydrofolate reductase for synthesis of tetrahydrofolate in Thermus thermophilus (2004), J. Bacteriol., 186, 351-355.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination and analysis, overexpression in Escherichia coli | Thermus thermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
x * 23000, recombinant enzyme, SDS-PAGE | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
gene DHTt | - |
| Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
gene DHTt | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme, partially from Escherichia coli, solubilization at pH 10.0 | Thermus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
recombinant enzyme, substrate dihydrofolate | Thermus thermophilus |
| 26.2 | - |
recombinant enzyme, substrate 6,7-dimethyl-7,8-dihydropteridine | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6,7-dimethyl-7,8-dihydropteridine + NADPH | - |
Thermus thermophilus | (6R)-L-erythro-6,7-dimethyl-5,6,7,8-tetrahydrobiopterin + NADP+ | - |
? |  |
| 6,7-dimethyl-7,8-dihydropteridine + NADPH | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | (6R)-L-erythro-6,7-dimethyl-5,6,7,8-tetrahydrobiopterin + NADP+ | - |
? | |
| dihydrofolate + NADPH | - |
Thermus thermophilus | tetrahydrofolate + NADP+ | - |
? | |
| dihydrofolate + NADPH | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | tetrahydrofolate + NADP+ | - |
? | |
| additional information | no activity with potassium ferricyanide, 6-biopterin, and 6-methyl-7,8-dihydropterine | Thermus thermophilus | ? | - |
? | |
| additional information | no activity with potassium ferricyanide, 6-biopterin, and 6-methyl-7,8-dihydropterine | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | x * 23000, recombinant enzyme, SDS-PAGE | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHPR | - |
Thermus thermophilus |
| dihydropteridine reductase | - |
Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Thermus thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | - |
Thermus thermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no flavin prosthetic group | Thermus thermophilus | |
| NADPH | - |
Thermus thermophilus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermus thermophilus | isoelectric focusing | 4.7 | 4.6 |
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