Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis, overexpression in Escherichia coli | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
x * 23000, recombinant enzyme, SDS-PAGE | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
gene DHTt | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
gene DHTt | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, partially from Escherichia coli, solubilization at pH 10.0 | Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.2 | - |
recombinant enzyme, substrate dihydrofolate | Thermus thermophilus |
26.2 | - |
recombinant enzyme, substrate 6,7-dimethyl-7,8-dihydropteridine | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,7-dimethyl-7,8-dihydropteridine + NADPH | - |
Thermus thermophilus | (6R)-L-erythro-6,7-dimethyl-5,6,7,8-tetrahydrobiopterin + NADP+ | - |
? | |
6,7-dimethyl-7,8-dihydropteridine + NADPH | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | (6R)-L-erythro-6,7-dimethyl-5,6,7,8-tetrahydrobiopterin + NADP+ | - |
? | |
dihydrofolate + NADPH | - |
Thermus thermophilus | tetrahydrofolate + NADP+ | - |
? | |
dihydrofolate + NADPH | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | tetrahydrofolate + NADP+ | - |
? | |
additional information | no activity with potassium ferricyanide, 6-biopterin, and 6-methyl-7,8-dihydropterine | Thermus thermophilus | ? | - |
? | |
additional information | no activity with potassium ferricyanide, 6-biopterin, and 6-methyl-7,8-dihydropterine | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 23000, recombinant enzyme, SDS-PAGE | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
DHPR | - |
Thermus thermophilus |
dihydropteridine reductase | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Thermus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | - |
Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no flavin prosthetic group | Thermus thermophilus | |
NADPH | - |
Thermus thermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermus thermophilus | isoelectric focusing | 4.7 | 4.6 |