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Literature summary for 1.5.1.34 extracted from
- NADPH-specific dihydropteridine reductase from bovine liver (1987), Methods Enzymol., 142, 111-116.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dichlorophenolindophenol | 0.0001 mM, 50% inhibition | Bos taurus |  |
| aminopterin | 1 mM, 50% inhibition, NADPH-specific enzyme | Bos taurus | |
| L-tyrosine | noncompetitive vs. NADPH | Bos taurus | |
| methopterin | 0.7 mM, 50% inhibition of NADPH-specific enzyme | Bos taurus | |
| NADP+ | competitive vs. NADPH | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0014 | - |
quinonoid 7,8-dihydro-6-methylpterin | cofactor NADPH, NADPH-specific enzyme | Bos taurus | |
| 0.0014 | - |
NADPH | reduction of quinonoid 7,8-dihydro-6-methylpterin, NADPH-specific enzyme | Bos taurus | |
| 0.0017 | - |
NADPH | reduction of quinonoid 7,8-dihydropterin, NADPH-specific enzyme | Bos taurus | |
| 0.0068 | - |
quinonoid 7,8-dihydropterin | cofactor NADPH, NADPH-specific enzyme | Bos taurus | |
| 0.013 | - |
quinonoid 7,8-dihydro-6-methylpterin | cofactor NADH, NADPH-specific enzyme | Bos taurus | |
| 2.9 | - |
NADH | reduction of quinonoid 7,8-dihydro-6-methylpterin, NADPH-specific enzyme | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, NADPH-specific dihydropteridine reductase, SDS-PAGE | Bos taurus |
| 65000 | - |
equilibrium centrifugation, NADPH-specific liver enzyme | Bos taurus |
| 68000 | - |
NADPH-specific enzyme, gel filtration | Bos taurus |
| 70000 | - |
NADPH-specific enzyme, native PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.972 | - |
NADPH-specific enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| quinonoid 6-methyl-7,8-(6H)-dihydropterin + NAD(P)H | - |
Bos taurus | 6-methyl-5,6,7,8-tetrahydropterin + NAD(P)+ | - |
? | |
| quinonoid 7,8-dihydropterin + NADPH + H+ | - |
Bos taurus | 5,6,7,8-tetrahydropterin + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35000, NADPH-specific dihydropteridine reductase, SDS-PAGE | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | liver: 2 distinct dihydropteridine reductases which catalyze the conversion of the quinonoid dihydropteridine to tetrahydropterin: NADH-dihydropteridine reductase, DPR, utilizes NADH as a better substrate than NADPH, NADPH-specific dihydropteridine reductase, TPR, shows strict specificity for NADPH | Bos taurus | |
| NADPH | liver: 2 distinct dihydropteridine reductases which catalyze the conversion of the quinonoid dihydropteridine to tetrahydropterin: NADH-dihydropteridine reductase, DPR, utilizes NADH as a better substrate than NADPH, NADPH-specific dihydropteridine reductase, TPR, shows strict specificity for NADPH | Bos taurus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0032 | - |
NADP+ | NADPH-specific enzyme | Bos taurus | |
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