Any feedback?
Literature summary for 1.5.1.34 extracted from
- Dihydropteridine reductase from bovine liver (1987), Methods Enzymol., 142, 103-110.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aminopterin | 0.025 mM, 50% inhibition of NADH-specific enzyme, 1 mM, 50% inhibition of NADPH-specific enzyme | Bos taurus |  |
| methopterin | 0.038 mM, 50% inhibition of NADH-specific enzyme | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00079 | - |
NADH | reduction of quinonoid 7,8-dihydro-6-methylpterin | Bos taurus | |
| 0.0011 | - |
quinonoid 7,8-dihydrobiopterin | cofactor NADPH | Bos taurus | |
| 0.0015 | - |
NADH | reduction of quinonoid 7,8-dihydrobiopterin | Bos taurus | |
| 0.0016 | - |
quinonoid 7,8-dihydrobiopterin | cofactor NADH | Bos taurus | |
| 0.0056 | - |
quinonoid 7,8-dihydro-6-methylpterin | cofactor NADH | Bos taurus | |
| 0.0058 | - |
quinonoid 7,8-dihydro-6-methylpterin | cofactor NADPH | Bos taurus | |
| 0.074 | - |
NADPH | reduction of quinonoid 7,8-dihydrobiopterin | Bos taurus | |
| 0.095 | - |
NADPH | reduction of quinonoid 7,8-dihydrobiopterin | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
2 * 25000, NADH-dihydropteridine reductase | Bos taurus |
| 49000 | 50000 | NADH-dihydropteridine reductase | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 70.7 | - |
NADH-specific enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| quinonoid 6-methyl-7,8-(6H)-dihydropterin + NAD(P)H | - |
Bos taurus | 6-methyl-5,6,7,8-tetrahydropterin + NAD(P)+ | - |
? | |
| quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | - |
Bos taurus | 5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? | |
| quinonoid dihydrobiopterin + NADH + H+ | - |
Bos taurus | 6R-(1'R,2'R)-5,6,7,8-tetrahydrobiopterin + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 25000, NADH-dihydropteridine reductase | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | utilizes both NADH and NADPH | Bos taurus | |
| NADH | enzyme prefers NADH | Bos taurus | |
| NADH | liver: 2 distinct dihydropteridine reductases which catalyze the conversion of the quinonoid dihydropteridine to tetrahydropterin: NADH-dihydropteridine reductase, DPR, utilizes NADH as a better substrate than NADPH, NADPH-specific dihydropteridine reductase, TPR, shows strict specificity for NADPH | Bos taurus | |
| NADPH | utilizes both NADH and NADPH | Bos taurus | |
| NADPH | liver: 2 distinct dihydropteridine reductases which catalyze the conversion of the quinonoid dihydropteridine to tetrahydropterin: NADH-dihydropteridine reductase, DPR, utilizes NADH as a better substrate than NADPH, NADPH-specific dihydropteridine reductase, TPR, shows strict specificity for NADPH | Bos taurus | |
| NADPH | NADH is preferred | Bos taurus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
