Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.34 extracted from

  • Kaufman, S.
    Dihydropteridine reductase from sheep liver (1987), Methods Enzymol., 142, 97-103.
    View publication on PubMed
Search for more literature for 1.5.1.34

Inhibitors

Inhibitors Comment Organism Structure
aminopterin
-
 Ovis aries
methotrexate
-
 Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 quinonoid dihydrobiopterin cofactor NADH Ovis aries
0.004
-
 NADH reduction of quinonoid dihydrobiopterin Ovis aries
0.0057
-
 NADH reduction of quinonoid 2-amino-4-hydroxy-6,7-dimetyldihydropteridine Ovis aries
0.006
-
 quinonoid 2-amino-4-hydroxy-6,7-dimethyldihydropteridine cofactor NADPH Ovis aries
0.009
-
 quinonoid dihydrobiopterin cofactor NADPH Ovis aries
0.0152
-
 2-amino-4-hydroxy-6,7-dimethyldihydropteridine cofactor NADH Ovis aries
0.0709
-
 NADPH reduction of quinonoid dihydrobiopterin Ovis aries
0.08
-
 NADPH reduction of quinonoid 2-amino-4-hydroxy-6,7-dimethyldihydropteridine Ovis aries

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 2 * 21000, liver, SDS-PAGE Ovis aries
41000
-
 gradient PAGE Ovis aries

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H Ovis aries essential component of the hepatic phenylalanine hydroxylating system 5,6,7,8-tetrahydrobiopterin + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ovis aries

Source Tissue

Source Tissue Comment Organism Textmining
adrenal medulla
-
 Ovis aries
-
brain
-
 Ovis aries
-
heart low activity Ovis aries
-
kidney
-
 Ovis aries
-
liver
-
 Ovis aries
-
skeletal muscle low activity Ovis aries
-
spleen low activity Ovis aries
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.6
-
-
 Ovis aries

Storage Stability

Storage Stability Organism
-80°C, stable for years Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-4-hydroxy-6,7-dimethyl-quinonoid-dihydropteridine + NAD(P)H
-
 Ovis aries 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine + NAD(P)+
-
 ?
quinonoid 6,7-dimethyl-7,8-(6H)-dihydropterin + NAD(P)H
-
 Ovis aries 6,7-dimethyl-5,6,7,8-tetrahydropterin + NAD(P)+
-
 ?
quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H absolutely specific for quinonoid dihydro-isomer corresponding to 7,8-dihydropterins Ovis aries 5,6,7,8-tetrahydrobiopterin + NAD(P)+
-
 ?
quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H essential component of the hepatic phenylalanine hydroxylating system Ovis aries 5,6,7,8-tetrahydrobiopterin + NAD(P)+
-
 ?
quinonoid dihydro-2-methylamino-4-hydroxy-6,7-dimethylpteridine + NAD(P)H approx. one-third of activity with 2-amino-4-hydroxy-6,7-dimethyl-quinonoid-dihydropterid Ovis aries tetrahydro-2-methylamino-4-hydroxy-6,7-dimethylpteridine + NAD(P)+
-
 ?
quinonoid dihydrobiopterin + NADH + H+
-
 Ovis aries 6R-(1'R,2'R)-5,6,7,8-tetrahydrobiopterin + NAD+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 21000, liver, SDS-PAGE Ovis aries

pH Range

pH Minimum pH Maximum Comment Organism
6.8 7.8 pH 6.8: about 45% activity, pH 7.8: about 15% activity Ovis aries

Cofactor

Cofactor Comment Organism Structure
NADH utilizes both NADH and NADPH Ovis aries
NADH enzyme prefers NADH Ovis aries
NADPH utilizes both NADH and NADPH Ovis aries
NADPH NADH is preferred Ovis aries

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.038
-
 methotrexate
-
 Ovis aries