Application | Comment | Organism |
---|---|---|
drug development | target for antifolate chemotherapy against Leishmania | Leishmania donovani |
Cloned (Comment) | Organism |
---|---|
expression of GFP-tagged enzyme in Leishmania donovani | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(4-fluoro-phenyl)-6-methyl-2-thioxo-1,2,3,4-tetrahydro-pyrimidine-5-carboxylic acid ethyl ester | - |
Leishmania donovani | |
2,6-dimethyl [3-O-benzyl-1,2-O isopropylidene-beta-L-threo-pentofuronose-4-yl]-1-phenyl-1,4-dihydro pyridine-3,5-dicarboxylic acid diethyl ether | - |
Leishmania donovani | |
sodium antimony gluconate | - |
Leishmania donovani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 biopterin + 3 NADPH + 3 H+ | Leishmania donovani | salvage of pterins, enzyme acts as a metabolic bypass for drugs targeting dihydrofolate reductase, PTR1 contributes about 10% of the reduction of folates in wild-type cells while the remaining 90% is due to the activity of dihydrofolate reductase-thymidylate synthase (EC 1.5.1.3) | dihydrobiopterin + tetrahydrobiopterin + 3 NADP+ | - |
? | |
folate + NADPH + H+ | Leishmania donovani | - |
7,8-dihydrofolate + tetrahydrofolate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | - |
clinical isolate | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 biopterin + 3 NADPH + 3 H+ | salvage of pterins, enzyme acts as a metabolic bypass for drugs targeting dihydrofolate reductase, PTR1 contributes about 10% of the reduction of folates in wild-type cells while the remaining 90% is due to the activity of dihydrofolate reductase-thymidylate synthase (EC 1.5.1.3) | Leishmania donovani | dihydrobiopterin + tetrahydrobiopterin + 3 NADP+ | - |
? | |
folate + NADPH + H+ | - |
Leishmania donovani | 7,8-dihydrofolate + tetrahydrofolate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pteridine reductase 1 | - |
Leishmania donovani |
PTR1 | - |
Leishmania donovani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Leishmania donovani |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
12.1 | - |
amastigote | Leishmania donovani | sodium antimony gluconate | |
18.3 | - |
amastigote | Leishmania donovani | 2,6-dimethyl [3-O-benzyl-1,2-O isopropylidene-beta-L-threo-pentofuronose-4-yl]-1-phenyl-1,4-dihydro pyridine-3,5-dicarboxylic acid diethyl ether | |
23.1 | - |
amastigote | Leishmania donovani | (4-fluoro-phenyl)-6-methyl-2-thioxo-1,2,3,4-tetrahydro-pyrimidine-5-carboxylic acid ethyl ester | |
90.8 | - |
promastigote | Leishmania donovani | 2,6-dimethyl [3-O-benzyl-1,2-O isopropylidene-beta-L-threo-pentofuronose-4-yl]-1-phenyl-1,4-dihydro pyridine-3,5-dicarboxylic acid diethyl ether | |
101 | - |
promastigote | Leishmania donovani | (4-fluoro-phenyl)-6-methyl-2-thioxo-1,2,3,4-tetrahydro-pyrimidine-5-carboxylic acid ethyl ester |