Literature summary for 1.5.1.30 extracted from

Jawanda, N.; Ebalunode, J.; Gribenko, A.; Briggs, J.; Lee, J.C.; Tu, S.C.
A single-residue mutation destabilizes Vibrio harveyi flavin reductase FRP dimer (2008), Arch. Biochem. Biophys., 472, 51-57.

Search for more literature for 1.5.1.30

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Vibrio harveyi

Protein Variants

Protein Variants	Comment	Organism
E99K	the mutation destabilizes the dimer and has an enhanced subunit dissociation as evident from a 44fold higher Kd for the monomer-dimer equilibrium	Vibrio harveyi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00007	-	NADPH	mutant enzyme E99K, in 50 mM phosphate buffer, at pH 7.0, at 23°C	Vibrio harveyi
0.002	-	FMN	mutant enzyme E99K, in 50 mM phosphate buffer, at pH 7.0, at 23°C	Vibrio harveyi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26300	-	2 * 26300	Vibrio harveyi

Organism

Organism	UniProt	Comment	Textmining
Vibrio harveyi	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ultracentrifugation, G-25 gel filtration, DEAE-Sepharose column chromatography, and Sephadex G25 gel filtration	Vibrio harveyi

Renatured (Commentary)

Renatured (Comment)	Organism
soluble denatured mutant enzyme E99K is mixed into 50fold volume of pre-cooled 50 mM phosphate buffer containing 0.1 M FMN and stirred on ice in the dark for 15 min for protein renaturation and the reconstitution of E99K holoenzyme	Vibrio harveyi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
FMN + NADPH	-	Vibrio harveyi	FMNH2 + NADP+	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 26300	Vibrio harveyi

Synonyms

Synonyms	Comment	Organism
FRP	-	Vibrio harveyi
NADPH-specific flavin reductase	-	Vibrio harveyi

Cofactor

Cofactor	Comment	Organism	Structure
FMN	contains one bound FMN cofactor per subunit	Vibrio harveyi
NADPH	-	Vibrio harveyi

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)