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Literature summary for 1.5.1.30 extracted from
- Altered mechanism of the alkanesulfonate FMN reductase with the monooxygenase enzyme (2005), Biochem. Biophys. Res. Commun., 331, 1137-1145.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Escherichia coli |  |
| NADPH | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000016 | - |
FMN | 25°C, single-enzyme assay | Escherichia coli | |
| 0.00013 | - |
FMN | 25°C, enzyme in complex with monooxygenase SsuD, presence of octanesulfonate | Escherichia coli | |
| 0.0054 | - |
NADPH | 25°C, single-enzyme assay | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
enzyme SsuE is part of alkanesulfonate monooxygenase system, together with a monooxygenase enzyme SsuD | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| reduced riboflavin + NADP+ = riboflavin + NADPH + H+ | single-enzyme kinetic assay, SsuE follows ordered sequential mechanism with NADPH as the first substrate to bind and NADP+ as the last product to leave. In presence of monooxygenase SsuD and octanesulfonate mechanism is altered to rapid equilibrium ordered mechanism, and Km-value for FMN increases 10fold | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FMN + NADPH | - |
Escherichia coli | FMNH2 + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alkanesulfonate FMN reductase | - |
Escherichia coli |
| SsuE | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
FMN | 25°C, enzyme in complex with monooxygenase SsuD, presence of octanesulfonate | Escherichia coli | |
| 1.9 | - |
FMN | 25°C, single-enzyme assay | Escherichia coli | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Escherichia coli | |
| NADPH | - |
Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0035 | - |
NADPH | 25°C, enzyme in complex with monooxygenase SsuD, presence of octanesulfonate | Escherichia coli | |
| 0.0039 | - |
NADPH | 25°C, single-enzyme assay | Escherichia coli | |
| 0.0139 | - |
NADP+ | 25°C, single-enzyme assay | Escherichia coli | |
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