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Literature summary for 1.5.1.30 extracted from

  • Lo, H.S.; Reeves, R.E.
    Purification and properties of NADPH: flavin oxidoreductase from Entamoeba histolytica (1980), Mol. Biochem. Parasitol., 2, 23-30.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
FMN
-
Entamoeba histolytica
0.01
-
FAD
-
Entamoeba histolytica
0.04
-
riboflavin
-
Entamoeba histolytica
0.04
-
galactoflavin
-
Entamoeba histolytica
0.05
-
NADPH at a saturating concentration of FMN Entamoeba histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
gel filtration Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
riboflavin + NADPH Entamoeba histolytica
-
reduced riboflavin + NADP+
-
?
riboflavin + NADPH Entamoeba histolytica NIH:200
-
reduced riboflavin + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
-
-
Entamoeba histolytica NIH:200
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Entamoeba histolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22
-
-
Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FAD + NADPH
-
Entamoeba histolytica FADH2 + NADP+
-
?
FAD + NADPH
-
Entamoeba histolytica NIH:200 FADH2 + NADP+
-
?
FMN + NADPH
-
Entamoeba histolytica FMNH2 + NADP+
-
?
FMN + NADPH
-
Entamoeba histolytica NIH:200 FMNH2 + NADP+
-
?
galactoflavin + NADPH
-
Entamoeba histolytica reduced galactoflavin + NADP+
-
?
galactoflavin + NADPH
-
Entamoeba histolytica NIH:200 reduced galactoflavin + NADP+
-
?
riboflavin + NADPH
-
Entamoeba histolytica reduced riboflavin + NADP+
-
?
riboflavin + NADPH
-
Entamoeba histolytica NIH:200 reduced riboflavin + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 38000-40000, SDS-PAGE Entamoeba histolytica

Synonyms

Synonyms Comment Organism
NADPH:flavin oxidoreductase
-
Entamoeba histolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
enzyme assay Entamoeba histolytica

Cofactor

Cofactor Comment Organism Structure
NADPH 20fold selectivity over NADH Entamoeba histolytica