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Literature summary for 1.5.1.26 extracted from
- Occurrence of beta-alanine-specific opine dehydrogenase in the muscle of the limpet Cellana grata Gould (Archaeogastropoda) (1999), Comp. Biochem. Physiol. B, 123B, 125-136.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.032 | - |
NADH | pH 7.1, 30°C | Cellana grata |  |
| 0.078 | - |
NAD+ | pH 9.0, 30°C | Cellana grata | |
| 0.19 | - |
pyruvate | pH 7.1, 30°C | Cellana grata | |
| 14.3 | - |
beta-Alanine | pH 7.1, 30°C | Cellana grata | |
| 35.2 | - |
beta-alanopine | pH 9.0, 30°C | Cellana grata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39700 | - |
gel filtration | Cellana grata |
| 40800 | - |
x * 40800, SDS-PAGE | Cellana grata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-alanine + pyruvate + NADH | Cellana grata | specific for beta-alanine | beta-alanopine + NAD+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellana grata | - |
Gold, isozyme beta-AlDH-2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by ammonium sulfate fractionation and several chromatographic steps, 705.6fold | Cellana grata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Cellana grata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Cellana grata |
| 13 | - |
purified enzyme, reverse reaction | Cellana grata |
| 190.5 | - |
purified enzyme, forward reaction | Cellana grata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-alanine + pyruvate + NADH | specific for beta-alanine | Cellana grata | beta-alanopine + NAD+ + H2O | - |
? | |
| additional information | tauropine, alanopine, strombine, and octopine are poor substrates | Cellana grata | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | x * 40800, SDS-PAGE | Cellana grata |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-AlDH-2 | - |
Cellana grata |
| More | the enzyme belongs to the opine dehydrogenase family of enzymes | Cellana grata |
| OpDH | - |
Cellana grata |
| opine dehydrogenase | - |
Cellana grata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Cellana grata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | - |
opine biosynthetic reaction direction | Cellana grata |
| 9 | - |
catabolic reaction direction | Cellana grata |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | half-maximal forward reaction at pH 5.8, half-maximal reverse reaction at pH 8.5 | Cellana grata |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Cellana grata | |
| NADH | - |
Cellana grata | |
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