General Stability | Organism |
---|---|
addition of 20% glycerol stabilizes the enzyme | Lactococcus lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N5-(L-1-carboxyethyl)-L-ornithine | - |
Lactococcus lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0066 | - |
NADPH | - |
Lactococcus lactis | |
0.15 | - |
pyruvate | - |
Lactococcus lactis | |
3.3 | - |
ornithine | - |
Lactococcus lactis | |
18.2 | - |
lysine | - |
Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | 38000 | immunoblot analysis | Lactococcus lactis |
38000 | - |
2 * 38000, SDS-PAGE | Lactococcus lactis |
38000 | - |
4 * 38000, SDS-PAGE | Lactococcus lactis |
39200 | - |
calculated from amino acid sequence | Lactococcus lactis |
78000 | - |
gel filtration | Lactococcus lactis |
150000 | - |
PAGE, hight pressure liquid chromatography, gel filtration | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
subsp. lactis K1, formerly Streptococcus lactis K1 | - |
Lactococcus lactis | - |
strains S.lactis K1 and 133 | - |
Purification (Comment) | Organism |
---|---|
- |
Lactococcus lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40.5 | - |
- |
Lactococcus lactis |
Storage Stability | Organism |
---|---|
-20°C, no significant loss of activity detected after 2 months of storage | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-ornithine + pyruvate + NADPH | pyruvic acid serves as an effective keto substrate for the condensation reaction | Lactococcus lactis | N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O | - |
ir | |
lysine + pyruvate + NADPH | - |
Lactococcus lactis | N7-(1-carboxyethyl)lysine + NADP+ + H2O | - |
ir | |
ornithine + 3-fluoropyruvate + NADPH | slight activity observed, about 15% of the rate found with pyruvate | Lactococcus lactis | N6-(1-carboxy-2-fluoroethyl)ornithine + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Lactococcus lactis |
tetramer | 4 * 38000, SDS-PAGE | Lactococcus lactis |
Synonyms | Comment | Organism |
---|---|---|
N5-CEO synthase | - |
Lactococcus lactis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | - |
Lactococcus lactis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable to heating for 10 min | Lactococcus lactis |
60 | - |
rapidly inactivated by heating at | Lactococcus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Lactococcus lactis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | - |
Lactococcus lactis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | high affinity for NADPH, NADH cannot replace NADPH, but nicotinamide hypoxanthine dinucleotide phosphate, reduced form, is an effective substitute for the natural cofactor | Lactococcus lactis |