Any feedback?
Literature summary for 1.5.1.22 extracted from
- Strombine dehydrogenase in the demosponge Suberites domuncula: characterization and kinetic properties of the enzyme crucial for anaerobic metabolism (2009), Comp. Biochem. Physiol. B, 154, 102-107.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| pyruvate | inhibited by an excess of substrate pyruvate; substrate inhibition, in excess, pyruvate enters in competition with the other substrate (glycine and/or NADH) preventing the site of reaction to take all the substrates to proceed with the enzymatic reaction. Its optimal concentration is determined to be round 2.0-2.3 mM | Suberites domuncula |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.34 | - |
pyruvate | pH 7.0, 30°C | Suberites domuncula | |
| 0.34 | - |
pyruvate | determined using constant cosubstrate concentrations (100 mM glycine and 0.3 mM NADH, pH 7.0) | Suberites domuncula | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
native PAGE | Suberites domuncula |
| 35000 | - |
semi-native PAGE | Suberites domuncula |
| 75000 | - |
native PAGE | Suberites domuncula |
| 75000 | - |
semi-native PAGE two bands at 35 kDa and 75 kDa are visible | Suberites domuncula |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Suberites domuncula | B1GT63 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.079 | - |
- |
Suberites domuncula |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gly + pyruvate + NADH | the enzyme is highly specific to glycine. No enzyme activity is observed when other amino acids (alanine and taurine) are used as substrates in the experiment | Suberites domuncula | D-Strombine + NAD+ + H2O | - |
? | |
| glycine + pyruvate + NADH | enzyme is specific for glycine | Suberites domuncula | D-strombine + NAD+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | native PAGE and semi-native PAGE, 2 * 35000 Da | Suberites domuncula |
| dimer | native and semi-native PAGE analysis, 2 * 35000 | Suberites domuncula |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| StDH | shows 68% sequence identity with the tauropine dehydrogenase protein from the marine demosponge Halichondria japonica | Suberites domuncula |
| strombine dehydrogenase | - |
Suberites domuncula |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Suberites domuncula |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Suberites domuncula |
| 7 | - |
assay at and optimal pH | Suberites domuncula |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Suberites domuncula | |
| NADH | enzyme shows an absolute requirement for NADH | Suberites domuncula | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
