Cloned (Comment) | Organism |
---|---|
cloning, disruption and expression of the two genes MET12 and MET13 encoding isoenzymes of MTHFR, MET12 is located on chromosome XVI and encodes a 657 amino acids protein, MET13 is located on chromosome VII, encodes a 599 amino acids protein and is responsible for most of the activity in cell, expression in Escherichia coli BL21 | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant strain RRY1 with single disruption of MET13 and strain RRY3 with double disruption of both MET12 and MET13, but not strain RRY2 with single disruption of MET12, result in loss of MTHFR activity and methionine auxothrophy, they can be complemented by overexpression of the human enzyme, but not by overexpression of the Escherichia coli metF gene | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
menadione | inhibits enzyme activity in yeast extracts | Saccharomyces cerevisiae | |
additional information | gene contains a putative S-adenosylmethionione binding sequence, that is inhibitory in eukaryotic MTHFR | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADPH | Saccharomyces cerevisiae | enzyme encoded by MET13 produces 5-methyltetrahydrofolate used for methylation of homocysteine for methionine biosynthesis in vivo, NADPH is the likely natural reductant | 5-methyltetrahydrofolate + NADP+ | - |
? | |
5,10-methylenetetrahydrofolate + NADPH | Saccharomyces cerevisiae DAY4 | enzyme encoded by MET13 produces 5-methyltetrahydrofolate used for methylation of homocysteine for methionine biosynthesis in vivo, NADPH is the likely natural reductant | 5-methyltetrahydrofolate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
two isoenzymes, encoded by the genes MET12 and MET13 | - |
Saccharomyces cerevisiae DAY4 | - |
two isoenzymes, encoded by the genes MET12 and MET13 | - |
Schizosaccharomyces pombe | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000094 | - |
wild-type, cell extract | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADPH | enzyme encoded by MET13 produces 5-methyltetrahydrofolate used for methylation of homocysteine for methionine biosynthesis in vivo, NADPH is the likely natural reductant | Saccharomyces cerevisiae | 5-methyltetrahydrofolate + NADP+ | - |
? | |
5,10-methylenetetrahydrofolate + NADPH | enzyme encoded by MET13 produces 5-methyltetrahydrofolate used for methylation of homocysteine for methionine biosynthesis in vivo, NADPH is the likely natural reductant | Saccharomyces cerevisiae DAY4 | 5-methyltetrahydrofolate + NADP+ | - |
? | |
5,10-methylenetetrahydrofolate + reduced acceptor | equilibrium lies far in favor of 5-methyltetrahydrofolate formation | Saccharomyces cerevisiae | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: NADPH as reduced acceptor | Saccharomyces cerevisiae | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
5,10-methylenetetrahydrofolate + reduced acceptor | equilibrium lies far in favor of 5-methyltetrahydrofolate formation | Saccharomyces cerevisiae DAY4 | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: NADPH as reduced acceptor | Saccharomyces cerevisiae DAY4 | 5-methyltetrahydrofolate + oxidized acceptor | - |
r | |
additional information | reverse reaction: menadione and 2,6-dichlorophenolindophenol and NADP+ are not effective as electron acceptor | Saccharomyces cerevisiae | ? | - |
? | |
additional information | reverse reaction: menadione and 2,6-dichlorophenolindophenol and NADP+ are not effective as electron acceptor | Saccharomyces cerevisiae DAY4 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | flavin-dependent enzyme | Saccharomyces cerevisiae |