Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADPH | substrate inhibition | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0128 | - |
NADPH | pH 7, 28°C | Trypanosoma cruzi | |
0.0277 | - |
1-pyrroline-5-carboxylate | pH 7, 28°C | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Trypanosoma cruzi | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q4DH60 | - |
- |
Trypanosoma cruzi CL Brener | Q4DH60 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-pyrroline-5-carboxylate + NADPH + H+ | - |
Trypanosoma cruzi | L-proline + NADP+ | - |
? | |
1-pyrroline-5-carboxylate + NADPH + H+ | - |
Trypanosoma cruzi CL Brener | L-proline + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P5CR | - |
Trypanosoma cruzi |
TcCLB.509207.90 | - |
Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
1-pyrroline-5-carboxylate | pH 7, 28°C | Trypanosoma cruzi | |
1.99 | - |
NADPH | pH 7, 28°C | Trypanosoma cruzi |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.045 | - |
pH 7, 28°C | Trypanosoma cruzi | NADPH |
General Information | Comment | Organism |
---|---|---|
physiological function | Trypanosoma cruzi biosynthesizes proline from 1-pyrroline-5-carboxylate, which is produced exclusively from glutamate. P5CR shows a higher expression in the insect-resident form of the parasite. The model that best explains the obtained data includes a non-competitive substrate inhibition mechanism | Trypanosoma cruzi |