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Literature summary for 1.5.1.2 extracted from

  • Forlani, G.; Petrollino, D.; Fusetti, M.; Romanini, L.; Nocek, B.; Joachimiak, A.; Berlicki, L.; Kafarski, P.
    DELTA1-pyrroline-5-carboxylate reductase as a new target for therapeutics: inhibition of the enzyme from Streptococcus pyogenes and effects in vivo (2012), Amino Acids, 42, 2283-2291.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.2

Inhibitors

Inhibitors Comment Organism Structure
([[3,5-bis(trifluoromethyl)phenyl]amino]methanediyl)bis(phosphonic acid) 71.2% inhibition at 0.2 mM Streptococcus pyogenes
[[(2,3-dichlorophenyl)amino]methanediyl]bis(phosphonic acid) 95.4% inhibition at 0.2 mM Streptococcus pyogenes
[[(2,4-dichlorophenyl)amino]methanediyl]bis(phosphonic acid) 52% inhibition at 0.2 mM Streptococcus pyogenes
[[(2,5-dichlorophenyl)amino]methanediyl]bis(phosphonic acid) 63.9% inhibition at 0.2 mM Streptococcus pyogenes
[[(2,6-dichlorophenyl)amino]methanediyl]bis(phosphonic acid) 96.2% inhibition at 0.2 mM Streptococcus pyogenes
[[(3,4-dichlorophenyl)amino]methanediyl]bis(phosphonic acid) 92.5% inhibition at 0.2 mM Streptococcus pyogenes
[[(3,5-dibromophenyl)amino]methanediyl]bis(phosphonic acid) 98.4% inhibition at 0.2 mM Streptococcus pyogenes
[[(3,5-dichlorophenyl)amino]methanediyl]bis(phosphonic acid) 91.1% inhibition at 0.2 mM Streptococcus pyogenes
[[(3,5-dimethylphenyl)amino]methanediyl]bis(phosphonic acid) 70.5% inhibition at 0.2 mM Streptococcus pyogenes
[[(3-chlorophenyl)amino]methanediyl]bis(phosphonic acid) 71.2% inhibition at 0.2 mM Streptococcus pyogenes
[[(4-benzylphenyl)amino]methanediyl]bis(phosphonic acid) 94.5% inhibition at 0.2 mM Streptococcus pyogenes
[[(4-chlorophenyl)amino]methanediyl]bis(phosphonic acid) 61.5% inhibition at 0.2 mM Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-pyrroline-5-carboxylate + NAD(P)H + H+ Streptococcus pyogenes
-
 L-proline + NAD(P)+
-
 ?
1-pyrroline-5-carboxylate + NAD(P)H + H+ Streptococcus pyogenes ATCC 19615
-
 L-proline + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes
-
-
-
Streptococcus pyogenes ATCC 19615
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity gel column chromatography Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-5-carboxylate + NAD(P)H + H+
-
 Streptococcus pyogenes L-proline + NAD(P)+
-
 ?
1-pyrroline-5-carboxylate + NAD(P)H + H+
-
 Streptococcus pyogenes ATCC 19615 L-proline + NAD(P)+
-
 ?
1-pyrroline-5-carboxylate + NADH + H+
-
 Streptococcus pyogenes L-proline + NAD+
-
 ?
1-pyrroline-5-carboxylate + NADH + H+
-
 Streptococcus pyogenes ATCC 19615 L-proline + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
DELTA1-pyrroline-5-carboxylate reductase
-
 Streptococcus pyogenes
P5C reductase
-
 Streptococcus pyogenes

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Streptococcus pyogenes
NADPH
-
 Streptococcus pyogenes

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00022
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(3,5-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
0.00022
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(3,5-dibromophenyl)amino]methanediyl]bis(phosphonic acid)
0.00039
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(3,4-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
0.00041
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(4-benzylphenyl)amino]methanediyl]bis(phosphonic acid)
0.00078
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes ([[3,5-bis(trifluoromethyl)phenyl]amino]methanediyl)bis(phosphonic acid)
0.00087
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(2,3-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
0.00088
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(2,6-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
0.0009
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(3-chlorophenyl)amino]methanediyl]bis(phosphonic acid)
0.0014
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(4-chlorophenyl)amino]methanediyl]bis(phosphonic acid)
0.0027
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(2,5-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
0.012
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(2,4-dichlorophenyl)amino]methanediyl]bis(phosphonic acid)
0.0256
-
 pH 7.5-8.0, at 37°C Streptococcus pyogenes [[(3,5-dimethylphenyl)amino]methanediyl]bis(phosphonic acid)