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Literature summary for 1.5.1.2 extracted from

  • Miler, P.A.; Stewart, C.R.
    Pyrroline-5-carboxylic acid reductase from soybean leaves (1976), Phytochemistry, 15, 1855-1857.
No PubMed abstract available

General Stability

General Stability Organism
sensitive to heat and low pH Glycine max

Inhibitors

Inhibitors Comment Organism Structure
ADP 1 mM Glycine max
ATP reversible with Mg2+ Glycine max
CTP 91% inactivation at 1 mM reversible with Mg2+ Glycine max
GTP 100% inactivation at 10 mM, reversible with Mg2+ Glycine max
Hg2+
-
Glycine max
NADP+
-
Glycine max
NH2OH
-
Glycine max
p-chloromercuribenzoate
-
Glycine max
phosphate
-
Glycine max
proline
-
Glycine max
thiazolidine-4-carboxylic acid
-
Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
NADPH
-
Glycine max
0.1
-
NADH
-
Glycine max
0.154
-
1-pyrroline-5-carboxylate
-
Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
60
-
-
Glycine max

Storage Stability

Storage Stability Organism
-10°C, 50% loss of activity in 4 weeks Glycine max
4°C, 50% loss of activity in 1 weeks Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-5-carboxylate + NADH + H+
-
Glycine max L-proline + NAD+
-
r
pyrroline-5-carboxylate + NAD(P)H + H+
-
Glycine max L-proline + NAD(P)+
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Glycine max

Cofactor

Cofactor Comment Organism Structure
NADH preferentially Glycine max
NADPH
-
Glycine max