KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
L-pyrroline-5-carboxylate | pH 8.0 | Clostridium sporogenes | |
0.33 | - |
L-pyrroline-5-carboxylate | pH 6.5 | Clostridium sporogenes | |
1 | 1.2 | NAD+ | - |
Clostridium sporogenes | |
1.25 | - |
L-proline | pH 10.2 | Clostridium sporogenes | |
31 | - |
L-proline | pH 8.0 | Clostridium sporogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium sporogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
copurification with proline oxidase, both activities located on the same protein | Clostridium sporogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
70.1 | - |
- |
Clostridium sporogenes |
Storage Stability | Organism |
---|---|
-18°C, 0.15-0.2 M potassium phosphate buffer pH 7.4, 2 months | Clostridium sporogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-pyrroline-5-carboxylate + NAD(P)H + H+ | specific for L-isomer | Clostridium sporogenes | L-proline + NAD(P)+ | - |
? | |
1-pyrroline-5-carboxylate + NADH + H+ | - |
Clostridium sporogenes | L-proline + NAD+ | - |
r | |
L-proline + NAD+ | - |
Clostridium sporogenes | 1-pyrroline-5-carboxylate + NADH + H+ | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, complete inactivation | Clostridium sporogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Clostridium sporogenes |
10.2 | - |
proline dehydrogenase activity | Clostridium sporogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Clostridium sporogenes | |
NADPH | no reaction | Clostridium sporogenes |