General Stability | Organism |
---|---|
dithiothreitol stabilizes during purification | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | 90% inhibition at 0.012 mM | Saccharomyces cerevisiae | |
N-ethylmaleimide | 90% inhibition at 0.02 mM, inhibition can be prevented by NADH | Saccharomyces cerevisiae | |
p-hydroxymercuribenzoate | 90% inhibition at 0.008 mM, inhibition can be prevented by addition of excess dithiothreitol | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
NADH | - |
Saccharomyces cerevisiae | |
0.056 | - |
NADPH | - |
Saccharomyces cerevisiae | |
0.08 | - |
DL-pyrroline-5-carboxylate | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
125000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
15 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, 0.1 mM dithiothreitol, stable for 6 months | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-pyrroline-5-carboxylate + NAD(P)H + H+ | - |
Saccharomyces cerevisiae | L-proline + NAD(P)+ | - |
? | |
1-pyrroline-5-carboxylate + NADH + H+ | - |
Saccharomyces cerevisiae | L-proline + NAD+ | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Saccharomyces cerevisiae |