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Literature summary for 1.5.1.17 extracted from

  • Storey, K.B.
    Tissue-specific alanopine dehydrogenase and strombine dehydrogenase from the sea mouse, Aphrodite aculeata (polychaeta) (1983), J. Exp. Zool., 225, 369-378.
No PubMed abstract available
Search for more literature for 1.5.1.17

Inhibitors

Inhibitors Comment Organism Structure
acetate
-
 Aphrodita aculeata
ATP
-
 Aphrodita aculeata
D-lactate strong Aphrodita aculeata
L-lactate strong Aphrodita aculeata
meso-Alanopine strong Aphrodita aculeata
NAD+ product inhibition Aphrodita aculeata
pyruvate substrate inhibition Aphrodita aculeata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 pyruvate
-
 Aphrodita aculeata
0.27
-
 pyruvate
-
 Aphrodita aculeata
0.75
-
 NAD+
-
 Aphrodita aculeata
0.9
-
 oxaloacetate
-
 Aphrodita aculeata
1.68
-
 meso-Alanopine
-
 Aphrodita aculeata
2.3
-
 Hydroxypyruvate
-
 Aphrodita aculeata
2.35
-
 2-oxobutanoate
-
 Aphrodita aculeata
2.75
-
 L-Ala
-
 Aphrodita aculeata
9
-
 NADH L-2-aminobutanoate Aphrodita aculeata
9.1
-
 2-oxopentanoate
-
 Aphrodita aculeata
12
-
 L-Cys
-
 Aphrodita aculeata
12.5
-
 L-Ala
-
 Aphrodita aculeata
25.5
-
 L-Thr
-
 Aphrodita aculeata
48
-
 L-Ser
-
 Aphrodita aculeata
70.5
-
 L-Val
-
 Aphrodita aculeata
285
-
 Gly
-
 Aphrodita aculeata
430
-
 Gly
-
 Aphrodita aculeata

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Aphrodita aculeata 5737
-
cytosol
-
 Aphrodita aculeata 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 gel filtration Aphrodita aculeata

Organism

Organism UniProt Comment Textmining
Aphrodita aculeata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Aphrodita aculeata

Source Tissue

Source Tissue Comment Organism Textmining
elytron
-
 Aphrodita aculeata
-
muscle longitudinal Aphrodita aculeata
-
nerve
-
 Aphrodita aculeata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
90
-
-
 Aphrodita aculeata

Storage Stability

Storage Stability Organism
4°C, stable for at least 1 week Aphrodita aculeata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly + pyruvate + NADH r Aphrodita aculeata Strombine + NAD+ + H2O
-
 ?
Gly + pyruvate + NADH at 161.5% of the activity with L-Ala Aphrodita aculeata Strombine + NAD+ + H2O
-
 ?
L-2-Aminobutyrate + pyruvate + NADH at 60% of the activity with L-Ala Aphrodita aculeata ?
-
 ?
L-Ala + 2-oxobutanoate + NADH at 21% of the activity with pyruvate Aphrodita aculeata ?
-
 ?
L-Ala + 2-oxopentanoate + NADH at 25.6% of the activity with pyruvate Aphrodita aculeata ?
-
 ?
L-Ala + hydroxypyruvate + NADH at 12.8% of the activity with pyruvate Aphrodita aculeata ?
-
 ?
L-Ala + oxaloacetate + NADH at 106.6% of the activity with pyruvate Aphrodita aculeata ?
-
 ?
L-Ala + pyruvate + NADH r Aphrodita aculeata 2,2'-Iminodipropanoate + NAD+ + H2O
-
 ?
L-Cys + pyruvate + NADH at 130.8% of the activity with L-Ala Aphrodita aculeata ?
-
 ?
L-Ser + pyruvate + NADH at 150.8% of the activity with L-Ala Aphrodita aculeata ?
-
 ?
L-Thr + pyruvate + NADH as effective as L-Ala Aphrodita aculeata ?
-
 ?
L-Val + pyruvate + NADH at 71.5% of the activity with L-Ala Aphrodita aculeata ?
-
 ?
meso-alanopine + NAD+ + H2O
-
 Aphrodita aculeata ?
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 with L-Ala and pyruvate as substrates Aphrodita aculeata
9
-
 with meso-alanopine as substrate Aphrodita aculeata

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Aphrodita aculeata
NADH
-
 Aphrodita aculeata