Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Saccharomyces cerevisiae | |
2,2'-bipyridine | - |
Saccharomyces cerevisiae | |
HgCl2 | - |
Saccharomyces cerevisiae | |
additional information | not: carbonyl reagents | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | - |
NAD+ | - |
Saccharomyces cerevisiae | |
2.32 | - |
L-saccharopine | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
1 * 50000, SDS-PAGE | Saccharomyces cerevisiae |
67000 | - |
gel filtration with Sephadex G-100 | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminoadipate 6-semialdehyde + L-glutamate + NADPH | Saccharomyces cerevisiae | lysine biosynthesis | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
baker's yeast | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | - |
Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.24 | - |
crude extract | Saccharomyces cerevisiae |
269.4 | - |
after purification | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-70°C, pH 8.0, 10 mM 2-mercaptoethanol, 5 mM EDTA | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminoadipate 6-semialdehyde + L-glutamate + NADPH | - |
Saccharomyces cerevisiae | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | r | |
2-aminoadipate 6-semialdehyde + L-glutamate + NADPH | lysine biosynthesis | Saccharomyces cerevisiae | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | - |
Saccharomyces cerevisiae | L-glutamate + (S)-2-amino-6-oxohexanoate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
34 | - |
denaturation above | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
saccharopine degradation | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | about 50% of activity maximum at pH 9 and 10 | Saccharomyces cerevisiae |