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Literature summary for 1.4.9.2 extracted from

  • Adachi, O.; Kubota, T.; Hacisalihoglu, A.; Toyama, H.; Shinagawa, E.; Duine, J.; Matsushita, K.
    Characterization of quinohemoprotein amine dehydrogenase from Pseudomonas putida (1998), Biosci. Biotechnol. Biochem., 62, 469-478.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
2,4-Dinitrophenylhydrazine
-
Pseudomonas putida
4-nitrophenylhydrazine
-
Pseudomonas putida
hydroxylamine
-
Pseudomonas putida
Semicarbazide
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
1 * 60000, 1 * 40000, 1 * 20000, SDS-PAGE Pseudomonas putida
40000
-
1 * 60000, 1 * 40000, 1 * 20000, SDS-PAGE Pseudomonas putida
60000
-
1 * 60000, 1 * 40000, 1 * 20000, SDS-PAGE Pseudomonas putida
120000
-
gel filtration and sedimentation equilibrium analysis Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P0A182 catalytic subunit
-
Pseudomonas putida IFO 15366 P0A182 catalytic subunit
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13
-
25°C, pH 8.0 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
n-amylamine + H2O + 2 blue protein
-
Pseudomonas putida ? + NH3 + 2 blue protein
-
?
n-amylamine + H2O + 2 blue protein
-
Pseudomonas putida IFO 15366 ? + NH3 + 2 blue protein
-
?
n-butylamine + H2O + 2 blue protein
-
Pseudomonas putida butanal + NH3 + 2 blue protein
-
?
n-butylamine + H2O + 2 blue protein
-
Pseudomonas putida IFO 15366 butanal + NH3 + 2 blue protein
-
?
propylamine + H2O + 2 blue protein the azurin-like blue protein is reduced specifically during AMDH reaction. The amine oxidation system can be reconstituted using AMDH, blue protein, and the cytoplasmic membranes of the organism Pseudomonas putida propanal + NH3 + 2 blue protein
-
?
propylamine + H2O + 2 blue protein the azurin-like blue protein is reduced specifically during AMDH reaction. The amine oxidation system can be reconstituted using AMDH, blue protein, and the cytoplasmic membranes of the organism Pseudomonas putida IFO 15366 propanal + NH3 + 2 blue protein
-
?

Subunits

Subunits Comment Organism
trimer 1 * 60000, 1 * 40000, 1 * 20000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
AMDH
-
Pseudomonas putida
quinohemoprotein amine dehydrogenase
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
heme c two mol of heme per mole of AMDH, 60 kDa subunit carries heme c. Typical absorption stectrum shows maxima at 554, 522, 420, and 320 nm in the reduced state and one peak at 410 nm, a shoulder at 350 nm, and a braod hill at 530 nm in the oxidized form Pseudomonas putida